20339-1-AP

antibody from Proteintech Group

Targeting: YBX1 BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

20339-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#20339-1-AP, RRID:AB_10665424

Product name

YBX1 antibody

Antibody type

Polyclonal

Description

KD/KO validated YBX1 antibody (Cat. #20339-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Isthmin-1 promotes growth and progression of colorectal cancer through the interaction with EGFR and YBX-1.

Zhou X, Zhang K, Wang C, Teng Y, Yu P, Cai W, Gao W, Li M, Ding Y, Sun P, Chen F, Wang Y, Ma J, Maeshige N, Ma X, Li Q, Liang X, Zhang Y, Su D
Cancer letters 2024 May 28;590:216868

---

RNA m(5)C modification upregulates E2F1 expression in a manner dependent on YBX1 phase separation and promotes tumor progression in ovarian cancer.

Liu X, Wei Q, Yang C, Zhao H, Xu J, Mobet Y, Luo Q, Yang D, Zuo X, Chen N, Yang Y, Li L, Wang W, Yu J, Xu J, Liu T, Yi P
Experimental & molecular medicine 2024 Mar;56(3):600-615

---

CDKL1 potentiates the antitumor efficacy of radioimmunotherapy by binding to transcription factor YBX1 and blocking PD-L1 expression in lung cancer.

Li Z, Xue H, Li J, Zheng Z, Liu Z, Dong X, Wang H, Chen J, Xu S
Journal of experimental & clinical cancer research : CR 2024 Mar 22;43(1):89

---

The m(6)A modification mediated-lncRNA POU6F2-AS1 reprograms fatty acid metabolism and facilitates the growth of colorectal cancer via upregulation of FASN.

Jiang T, Qi J, Xue Z, Liu B, Liu J, Hu Q, Li Y, Ren J, Song H, Xu Y, Xu T, Fan R, Song J
Molecular cancer 2024 Mar 16;23(1):55

---

Mechanism of Endogenous Peptide PDYBX1 and Precursor Protein YBX1 in Hirschsprung's Disease.

Sun Q, Zhi Z, Wang C, Du C, Tang J, Li H, Tang W
Neuroscience bulletin 2024 Jun;40(6):695-706

---

Stabilization of KPNB1 by deubiquitinase USP7 promotes glioblastoma progression through the YBX1-NLGN3 axis.

Li J, Zhang B, Feng Z, An D, Zhou Z, Wan C, Hu Y, Sun Y, Wang Y, Liu X, Wei W, Yang X, Meng J, Che M, Sheng Y, Wu B, Wen L, Huang F, Li Y, Yang K
Journal of experimental & clinical cancer research : CR 2024 Jan 23;43(1):28

---

LncRNA LY6E-DT and its encoded metastatic-related protein play oncogenic roles via different pathways and promote breast cancer progression.

Liu HT, Gao ZX, Li F, Guo XY, Li CL, Zhang H, Zhao RN, Liu Y, Shi DB, Zhu WJ, Gao P
Cell death and differentiation 2024 Feb;31(2):188-202

---

MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing.

Vital T, Wali A, Butler KV, Xiong Y, Foster JP 2nd, Marcel SS, McFadden AW, Nguyen VU, Bailey BM, Lamb KN, James LI, Frye SV, Mosely AL, Jin J, Pattenden SG, Davis IJ
Frontiers in oncology 2023;13:1099550

---

Knockdown of Y-box binding protein 1 induces autophagy in early porcine embryos.

Jiang WJ, Lee SH, Heo G, Chung HJ, Cho ES, Sa SJ, Hochi S, Cui XS
Frontiers in cell and developmental biology 2023;11:1238546

---

YBX1 as an oncogenic factor in T-cell acute lymphoblastic leukemia.

Li H, Zhang D, Fu Q, Wang S, Wang Z, Zhang X, Chen X, Zhu X, An N, Chen Y, Zhou L, Lu D, Zhao N
Blood advances 2023 Sep 12;7(17):4874-4885

---

A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability.

Li K, Guo J, Ming Y, Chen S, Zhang T, Ma H, Fu X, Wang J, Liu W, Peng Y
Nature communications 2023 Oct 28;14(1):6876

---

Silencing circSERPINE2 restrains mesenchymal stem cell senescence via the YBX3/PCNA/p21 axis.

Chen F, Wang S, Zeng C, Tang S, Gu H, Wang Z, Li J, Feng P, Zhang Y, Wang P, Wu Y, Shen H
Cellular and molecular life sciences : CMLS 2023 Oct 13;80(11):325

---

Long non-coding RNA KCND1 protects hearts from hypertrophy by targeting YBX1.

Yang R, Li L, Hou Y, Li Y, Zhang J, Yang N, Zhang Y, Ji W, Yu T, Lv L, Liang H, Li X, Li T, Shan H
Cell death & disease 2023 May 30;14(5):344

---

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation.

Curdy N, Lanvin O, Cerapio JP, Pont F, Tosolini M, Sarot E, Valle C, Saint-Laurent N, Lhuillier E, Laurent C, Fournié JJ, Franchini DM
Cell reports 2023 Mar 28;42(3):112211

---

Y-box binding protein 1 influences zygotic genome activation by regulating N6-methyladenosine in porcine embryos.

Jiang WJ, Sun MH, Li XH, Lee SH, Heo G, Zhou D, Cui XS
Journal of cellular physiology 2023 Jul;238(7):1592-1604

---

THOC3 interacts with YBX1 to promote lung squamous cell carcinoma progression through PFKFB4 mRNA modification.

Yu T, Zhang Q, Yu SK, Nie FQ, Zhang ML, Wang Q, Lu KH
Cell death & disease 2023 Jul 27;14(7):475

---

TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential.

He J, Lin M, Zhang X, Zhang R, Tian T, Zhou Y, Dong W, Yang Y, Sun X, Dai Y, Xu Y, Zhang Z, Xu M, Lei QY, Xu Y, Lv L
Cell discovery 2023 Aug 8;9(1):84

---

YB-1 is a positive regulator of KLF5 transcription factor in basal-like breast cancer.

Jiang D, Qiu T, Peng J, Li S, Tala, Ren W, Yang C, Wen Y, Chen CH, Sun J, Wu Y, Liu R, Zhou J, Wu K, Liu W, Mao X, Zhou Z, Chen C
Cell death and differentiation 2022 Jun;29(6):1283-1295

---

CTPS1 promotes malignant progression of triple-negative breast cancer with transcriptional activation by YBX1.

Lin Y, Zhang J, Li Y, Guo W, Chen L, Chen M, Chen X, Zhang W, Jin X, Jiang M, Xiao H, Wang C, Song C, Fu F
Journal of translational medicine 2022 Jan 6;20(1):17

---

Coordinated post-transcriptional control of oncogene-induced senescence by UNR/CSDE1.

Avolio R, Inglés-Ferrándiz M, Ciocia A, Coll O, Bonnin S, Guitart T, Ribó A, Gebauer F
Cell reports 2022 Jan 11;38(2):110211

---

TGF-β/YB-1/Atg7 axis promotes the proliferation of hepatic progenitor cells and liver fibrogenesis.

Guo Y, Zhu J, Xu X, Shen B, Shen Z, Li B, Li F, Gu T, Cai X, Dong H, Lu L
Biochimica et biophysica acta. Molecular basis of disease 2022 Jan 1;1868(1):166290

---

Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition.

Zhang C, Wang M, Li Y, Zhang Y
Science advances 2022 Feb 4;8(5):eabj3967

---

LncRNA ELF3-AS1 inhibits gastric cancer by forming a negative feedback loop with SNAI2 and regulates ELF3 mRNA stability via interacting with ILF2/ILF3 complex.

Li D, Shen L, Zhang X, Chen Z, Huang P, Huang C, Qin S
Journal of experimental & clinical cancer research : CR 2022 Dec 2;41(1):332

---

Selective sorting and secretion of hY4 RNA fragments into extracellular vesicles mediated by methylated YBX1 to promote lung cancer progression.

Li C, Wang W, Sun Y, Ni Y, Qin F, Li X, Wang T, Guo M, Sun G
Journal of experimental & clinical cancer research : CR 2022 Apr 11;41(1):136

---

hTERT Promotes CRC Proliferation and Migration by Recruiting YBX1 to Increase NRF2 Expression.

Gong C, Yang H, Wang S, Liu J, Li Z, Hu Y, Chen Y, Huang Y, Luo Q, Wu Y, Liu E, Xiao Y
Frontiers in cell and developmental biology 2021;9:658101

---

Effects of Y Box Binding Protein-1 in Progression and Prognosis of Cholangiocarcinoma.

Chen Y, Jiang P, Tian F, Chen G, Peng T, Deng X, Li Z, Huang D, Zhu J, Zhen P, Xie C, Wang S
Journal of investigative surgery : the official journal of the Academy of Surgical Research 2021 Jan;34(1):55-63

---

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Lei H, Xu HZ, Shan HZ, Liu M, Lu Y, Fang ZX, Jin J, Jing B, Xiao XH, Gao SM, Gao FH, Xia L, Yang L, Liu LG, Wang WW, Liu CX, Tong Y, Wu YZ, Zheng JK, Chen GQ, Zhou L, Wu YL
Nature communications 2021 Jan 4;12(1):51

---

Long noncoding RNA SNHG6 promotes carcinogenesis by enhancing YBX1-mediated translation of HIF1α in clear cell renal cell carcinoma.

Zhao P, Deng Y, Wu Y, Guo Q, Zhou L, Yang X, Wang C
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Feb;35(2):e21160

---

Proteomic Profile of Sperm in Infertile Males Reveals Changes in Metabolic Pathways.

Liang J, Zheng Y, Zeng W, Chen L, Yang S, Du P, Wang Y, Yu X, Zhang X
The protein journal 2021 Dec;40(6):929-939

---

A reciprocal feedback of miR-548ac/YB-1/Snail induces EndMT of HUVECs during acidity microenvironment.

Chen J, Han S, Chen J, Hu P, Zeng Z, Hu Y, Xiong H, Ke Z, Zhang Y, Xu F, Zhao G
Cancer cell international 2021 Dec 20;21(1):692

---

The long noncoding RNA AATBC promotes breast cancer migration and invasion by interacting with YBX1 and activating the YAP1/Hippo signaling pathway.

Wang M, Dai M, Wang D, Tang T, Xiong F, Xiang B, Zhou M, Li X, Li Y, Xiong W, Li G, Zeng Z, Guo C
Cancer letters 2021 Aug 1;512:60-72

---

Comparison of proteomic profiles from the testicular tissue of males with impaired and normal spermatogenesis.

Liang J, Zheng Y, Zeng W, Chen L, Yang S, Du P, Wang Y, Yu X, Zhang X
Systems biology in reproductive medicine 2021 Apr;67(2):127-136

---

Enhanced YB1/EphA2 axis signaling promotes acquired resistance to sunitinib and metastatic potential in renal cell carcinoma.

Ruan H, Li S, Bao L, Zhang X
Oncogene 2020 Sep;39(38):6113-6128

---

Y-box binding protein 1 acts as a negative regulator of stearoyl CoA desaturase 1 in clear cell renal cell carcinoma.

Jeffords E, Freeman S, Cole B, Root K, Chekouo T, Melvin RG, Bemis L, Simmons GE Jr
Oncology letters 2020 Nov;20(5):165

---

LncRNA LINC00472 regulates cell stiffness and inhibits the migration and invasion of lung adenocarcinoma by binding to YBX1.

Deng X, Xiong W, Jiang X, Zhang S, Li Z, Zhou Y, Xiang B, Zhou M, Li X, Li G, Zeng Z, Gong Z
Cell death & disease 2020 Nov 3;11(11):945

---

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

Ruan H, Song Z, Cao Q, Ni D, Xu T, Wang K, Bao L, Tong J, Xiao H, Xiao W, Cheng G, Xiong Z, Liang H, Liu D, Wang L, Olivier T, Jane BH, Yang H, Zhang X, Chen K
Molecular therapy : the journal of the American Society of Gene Therapy 2020 May 6;28(5):1299-1313

---

CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA-Binding Proteins and Sponging MiR-942.

Wong CH, Lou UK, Li Y, Chan SL, Tong JH, To KF, Chen Y
Cancer research 2020 Jun 1;80(11):2138-2149

---

Systematic quantitative analysis of ribosome inventory during nutrient stress.

An H, Ordureau A, Körner M, Paulo JA, Harper JW
Nature 2020 Jul;583(7815):303-309

---

SWI/SNF complex subunit BAF60a represses hepatic ureagenesis through a crosstalk between YB-1 and PGC-1α.

Zhang W, Dong Z, Xu M, Zhang S, Liu C, Chen S
Molecular metabolism 2020 Feb;32:85-96

---

Peptidome characterization of ovarian cancer serum and the identification of tumor suppressive peptide ZYX(36-58).

Wang X, Liu G, Sheng N, Zhang M, Pan X, Liu S, Huang K, Cong Y, Xu Q, Jia X, Xu J
Annals of translational medicine 2020 Aug;8(15):925

---

Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis.

He X, Wang S, Li M, Zhong L, Zheng H, Sun Y, Lai Y, Chen X, Wei G, Si X, Han Y, Huang S, Li X, Liao W, Liao Y, Bin J
Theranostics 2019;9(19):5558-5576

---

Targeting the YB-1/PD-L1 Axis to Enhance Chemotherapy and Antitumor Immunity.

Tao Z, Ruan H, Sun L, Kuang D, Song Y, Wang Q, Wang T, Hao Y, Chen K
Cancer immunology research 2019 Jul;7(7):1135-1147

---

PTBP3 contributes to colorectal cancer growth and metastasis via translational activation of HIF-1α.

Hou P, Chen F, Yong H, Lin T, Li J, Pan Y, Jiang T, Li M, Chen Y, Song J, Zheng J, Bai J
Journal of experimental & clinical cancer research : CR 2019 Jul 10;38(1):301

---

Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.

Shelkovnikova TA, An H, Skelt L, Tregoning JS, Humphreys IR, Buchman VL
Cell reports 2019 Dec 24;29(13):4496-4508.e4

---

Stress granules regulate stress-induced paraspeckle assembly.

An H, Tan JT, Shelkovnikova TA
The Journal of cell biology 2019 Dec 2;218(12):4127-4140

---

YB-1 regulates tiRNA-induced Stress Granule formation but not translational repression.

Lyons SM, Achorn C, Kedersha NL, Anderson PJ, Ivanov P
Nucleic acids research 2016 Aug 19;44(14):6949-60

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 20339-1-AP(YBX1 antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human placenta using 20339-1-AP(YBX1 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The YBX1 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human YBX1. This antibody recognizes human, mouse antigen. The YBX1 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

20339-1-AP.pdf

Show more