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LearnGTX62722
antibody from GeneTex
Targeting: YBX1
BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- GTX62722 - Provider product page
- Provider
- GeneTex
- Product name
- YB1 antibody [EP2708Y]
- Antibody type
- Monoclonal
- Antigen
- A synthetic peptide corresponding to residues near the C terminus of human YB1
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100µl
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis on (A) HeLa (B) SW480 (C) A549 (D) MCF-7 cell lysates using anti-YB-1 rabbit mAb (cat. # GTX62722), dilution 1:200,000.
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WB analysis of (1) HeLa, (2) SW480, (3) A549 and (4) MCF7 lysates (10 ?g per lane) using YB1 antibody [EP2708Y] at a dilution of 1:200,000.
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IP was perform with 0.5 mg of Hek293 extract and 10 ?g of YB1 antibody [EP2708Y] (+). No antibody was added to the control (-). The precipitates were detected by YB1 antibody [EP2708Y].
- Validation comment
- IP
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- FACS analysis of permeabilized HeLa cells using YB1 antibody [EP2708Y] at a dilution of 1:100 (red) or an isotype control (black).
- Validation comment
- FACS
