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LearnPA5-96766
antibody from Invitrogen Antibodies
Targeting: YBX1
BP-8, CSDA2, CSDB, DBPB, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [5]
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- Product number
- PA5-96766 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- YBX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: LRSVGDGETV EFDVVEGEKG AEAANVTGPG GVPVQGSKYA ADRNHYRRYP RRRGPPRNYQ QNYQNSESGE KNEGSESAPE GQAQQRRPYR RRRFPPYYMR R; Positive Samples: THP-1, Mouse heart, Rat heart; Cellular Location: Cytoplasm, Cytoplasmic granule, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.22 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using YBX1 Polyclonal antibody (Product # PA5-96766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of YBX1 in extracts from normal (control) and YBX1 knockout (KO) HeLa cells using YBX1 Polyclonal Antibody (Product # PA5-96766) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of YBX1 in extracts of HepG2 cells using YBX1 Polyclonal Antibody (Product # PA5-96766) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of YBX1 in paraffin-embedded human colon carcinoma using YBX1 Polyclonal Antibody (Product # PA5-96766) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of YBX1 in paraffin-embedded mouse testis using YBX1 Polyclonal Antibody (Product # PA5-96766) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of YBX1 was performed in mouse colon tissue using YBX1 Polyclonal Antibody (Product # PA5-96766) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of YBX1 was performed in mouse colone artery tissue using YBX1 Polyclonal Antibody (Product # PA5-96766) at a dilution of 1:100. Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry-Immunofluorescence analysis of YBX1 was performed in mouse brain tissue using YBX1 Polyclonal Antibody (Product # PA5-96766) at a dilution of 1:100. Blue: DAPI for nuclear staining.
