MA5-31712

antibody from Invitrogen Antibodies

Targeting: DDX1 DBP-RB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31712

Provider

Invitrogen Antibodies

Product name

DDX1 Monoclonal Antibody (3E5E2)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3E5E2

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDX1 in HeLa (1), MCF-7 (2), A431 (3), PC-3 (4), NIH/3T3 (5), Jurkat (6), U251 (7), and HEK293 (8) cell lysate. Samples were incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDX1 in human DDX1 recombinant protein. Sample was incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:500-1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDX1 in HEK293 (1) and DDX1 cell lysate. Samples were incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:500-1:2000.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of DDX1 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of DDX1 in Hela cells (green). Sample was incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DDX1 in paraffin-embedded colon cancer tissue. Sample was incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:200-1:1000 followed by DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DDX1 in paraffin-embedded bladder cancer tissue. Sample was incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of DDX1 in HeLa cells (green). Sample was incubated with DDX1 monoclonal antibody (Product # MA5-31712) using a dilution of 1:200-1:400 followed by negative control (red).