PA5-93070

antibody from Invitrogen Antibodies

Targeting: DDX1 DBP-RB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93070

Provider

Invitrogen Antibodies

Product name

DDX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VHHVVVPVNP KTDRLWERLG KSHIRTDDVH AKDNTRPGAN SPEMWSEAIK ILKGEYAVRA IKEHKMDQAI IFCRTKIDCD NLEQYFIQQG GGPDKKGHQF SCVCLHGDRK PHERKQNLER FKKGDVRFLI CTDVAARGID IHGVPYVINV TLPDEKQNYV HRIGRVGRAE RMGLAISLVA TEKEKVWYHV CSSRGKGCYN TRLKEDGGCT IWYNEMQLLS EIEEHLNCTI SQVEPDIKVP VDEFDGKVTY GQKRAAGGGS YKGHVDILAP TVQELAALEK EAQTSFLHLG YLPNQLFRTF; Positive Samples: MCF7, HepG2, HeLa, 293T, U-251MG, Mouse brain, Mouse skeletal muscle, Rat brain, Rat skeletal muscle; Cellular Location: Cytoplasm, Cytoplasmic granule, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.33 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DDX1 in 293F cells. Samples were incubated with DDX1 Polyclonal antibody (Product # PA5-93070) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of DDX1 was performed in MCF7 cells using DDX1 Polyclonal Antibody (Product # PA5-93070).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of DDX1 in HeLa cells. Samples were incubated with DDX1 Polyclonal antibody (Product # PA5-93070). Blue: DAPI for nuclear staining.

Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of DDX1 in 300 μg extracts of 293F cells. Samples were precipitated with 3 μg DDX1 Polyclonal antibody (Product # PA5-93070). Western blot was performed from the immunoprecipitate using DDX1 Polyclonal antibody (Product # PA5-93070) at a dilution of 1:2,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DDX1 in paraffin-embedded rat brain. Samples were incubated with DDX1 Polyclonal antibody (Product # PA5-93070) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DDX1 in paraffin-embedded mouse lung. Samples were incubated with DDX1 Polyclonal antibody (Product # PA5-93070) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DDX1 in paraffin-embedded mouse brain. Samples were incubated with DDX1 Polyclonal antibody (Product # PA5-93070) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of DDX1 in paraffin-embedded human leiomyoma of uterus. Samples were incubated with DDX1 Polyclonal antibody (Product # PA5-93070) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of DDX1 was performed in 100 µg extracts of 293T cells using DDX1 Polyclonal Antibody (Product # PA5-93070). Western blot was performed from the immunoprecipitate using DDX1 Polyclonal Antibody at a dilution of 1:1000.