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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28210 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DDX1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2. Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (89%), Xenopus laevis (88%), Chicken (93%), Rhesus Monkey (98%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.76 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DDX1 Polyclonal Antibody (Product # PA5-28210). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. 7.5% SDS PAGE. DDX1 Polyclonal Antibody (Product # PA5-28210) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DDX1 in methanol-fixed HeLa cells using a DDX1 polyclonal antibody (Product # PA5-28210) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDX1 Polyclonal Antibody detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. DDX1 Polyclonal Antibody (Product # PA5-28210) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DDX1 Polyclonal Antibody detects DDX1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. DDX1 Polyclonal Antibody (Product # PA5-28210) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
