PA5-28210

antibody from Invitrogen Antibodies

Targeting: DDX1 DBP-RB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28210

Provider

Invitrogen Antibodies

Product name

DDX1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A431, H1299, HeLa, HepG2.

Concentration

0.76 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DDX1 Polyclonal Antibody (Product # PA5-28210). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. 7.5% SDS PAGE. DDX1 Polyclonal Antibody (Product # PA5-28210) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of DDX1 in methanol-fixed HeLa cells using a DDX1 polyclonal antibody (Product # PA5-28210) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DDX1 Polyclonal Antibody detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. DDX1 Polyclonal Antibody (Product # PA5-28210) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DDX1 Polyclonal Antibody detects DDX1 protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. DDX1 Polyclonal Antibody (Product # PA5-28210) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.