Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- IC4470F - Provider product page
- Provider
- R&D Systems
- Product name
- Human Myosin Heavy Chain Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects Myosin Heavy Chain in human, mouse, rat and other mammalian, avian, and amphibian species.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MF20
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references MAP4K4 Inhibition Promotes Survival of Human Stem Cell-Derived Cardiomyocytes and Reduces Infarct Size In Vivo.
APOBEC2 negatively regulates myoblast differentiation in muscle regeneration.
Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.
Fiedler LR, Chapman K, Xie M, Maifoshie E, Jenkins M, Golforoush PA, Bellahcene M, Noseda M, Faust D, Jarvis A, Newton G, Paiva MA, Harada M, Stuckey DJ, Song W, Habib J, Narasimhan P, Aqil R, Sanmugalingam D, Yan R, Pavanello L, Sano M, Wang SC, Sampson RD, Kanayaganam S, Taffet GE, Michael LH, Entman ML, Tan TH, Harding SE, Low CMR, Tralau-Stewart C, Perrior T, Schneider MD
Cell stem cell 2019 Apr 4;24(4):579-591.e12
Cell stem cell 2019 Apr 4;24(4):579-591.e12
APOBEC2 negatively regulates myoblast differentiation in muscle regeneration.
Ohtsubo H, Sato Y, Suzuki T, Mizunoya W, Nakamura M, Tatsumi R, Ikeuchi Y
The international journal of biochemistry & cell biology 2017 Apr;85:91-101
The international journal of biochemistry & cell biology 2017 Apr;85:91-101
Calpain 3 deficiency affects SERCA expression and function in the skeletal muscle.
Toral-Ojeda I, Aldanondo G, Lasa-Elgarresta J, Lasa-Fernández H, Fernández-Torrón R, López de Munain A, Vallejo-Illarramendi A
Expert reviews in molecular medicine 2016 Apr 8;18:e7
Expert reviews in molecular medicine 2016 Apr 8;18:e7
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Myosin Heavy Chain in C2C12 Mouse Cell Line by Flow Cytometry. Three-day differentiated C2C12 mouse myoblast cell line was stained with Mouse Anti-Human Myosin Heavy Chain Fluorescein-conjugated Monoclonal Antibody (Catalog # IC4470F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
