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Learn66825-1-IG
antibody from Invitrogen Antibodies
Targeting: MYH14
DFNA4, FLJ13881, KIAA2034, MHC16, MYH17
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- 66825-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYH14 Monoclonal Antibody (1B4C9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MQARRGKGA WEKTRLALEA EVSELRAELS SLQTARQEGE QRRRRLELQL QEVQGRAGDG ERARAEAAEK LQRAQAELEN VSGALNEAES KTIRLSKELS STEAQLHDAQ ELLQEETRAK LALGSRVRAM EAEAAGLREQ LEEEAAARER AGRELQTAQA QLSEWRRRQE EEAGALEAGE EARRRAAREA EALTQRLAEK TE (1231-1430 aa encoded by NM_001077186)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B4C9
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-29 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded mouse small intestine tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 66825-1-IG (MYH14 antibody) at dilution of 1:2000 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon tissue slide using 66825-1-IG (MYH14 antibody) at dilution of 1:2000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HT-29 cells were intracellularly stained with 0.2 µg Anti-Human MYH14 (Product # 66825-1-IG, Clone:1B4C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.
