TA308895

antibody from OriGene

Targeting: H3-3B H3.3B, H3F3B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA308895

Provider

OriGene

Product name

Rabbit polyclonal anti-H3F3B(Histone H3) antibody, Loading control

Antibody type

Polyclonal

Description

Rabbit polyclonal anti-H3F3B(Histone H3) antibody, Loading control

Host

Rabbit

Conjugate

Unconjugated

Epitope

H3F3B

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

0.44mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Sample (50 ug of whole cell lysate). A: mouse brain. 15% SDS PAGE. TA308895 diluted at 1:1000.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Histone H3.3B antibody detects H3F3B protein by Western blot analysis. A. 50 ug rat brain lysate/extract. 15 % SDS-PAGE. Histone H3.3B antibody (TA308895) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate). A: Hela. 15% SDS PAGE. TA308895 diluted at 1:1000.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3.3B protein stained by Histone H3.3B antibody (TA308895) diluted at 1:50

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H3.3B(TA308895) antibody at 1:200 dilution.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Histone H3.3B antibody (TA308895) dilution: 1:500.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Histone H3.3B antibody (TA308895) dilution: 1:500.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(TA308895) antibody at 1:500 dilution.

Validation comment

IHC