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antibody from NSJ Bioreagents
Targeting: H3-3B
H3.3B, H3F3B
Western blot ELISA Immunocytochemistry
Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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Validation data
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- Product number
- R20208 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant H3K9ac Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant H3K9ac antibody is suitable for use in Western blot/ELISA/ChImmunoprecipitation/Immunocytochemistry/Immunohistochemistry applications with all species samples.
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM161
- Vial size
- 25 ug, 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant H3K9ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K9ac antibody at 0.25 ug/ml showed a band of Histone H3 acetylated at Lysine 9 in treated HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with acetylated Histone H3 at Lys 9 using HeLa whole cell lysate, treated or untreated with sodium butyrate, anti-H3CT (RM188, 1 ug/ml) as the capture and biotinylated recombinant H3K9ac antibody (RM161, 1 ug/ml) as the detect.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF testing of HeLa cells treated with sodium butyrate using recombinant H3K9ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of human HepG2 cells with recombinant H3K9ac antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ChIP performed on HeLa cells using recombinant H3K9ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- The recombinant H3K9ac antibody specifically reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetylated Lysine 4/14/18/23/27/36/56/79/122 in Histone H3.
