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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [2]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- R20209 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant H3K18ac Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant H3K18ac antibody is suitable for use in Western blot/ELISA/ChImmunoprecipitation/Immunocytochemistry. Immunohistochemistry applications with all species samples.
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM166
- Vial size
- 25 ug, 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant H3K18ac antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of acid extracts from HeLa cells untreated (-) or treated (+) with sodium butyrate using recombinant H3K18ac antibody at 0.5 ug/mL showed a band of Histone H3 acetylated at Lysine 18 in treated HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA with acetylated Histone H3 at Lys 18 using human HeLa cell lysate, treated or untreated with sodium butyrate, using anti-Histone H3CT (RM188, 1 ug/ml) as the capture and biotinylated recombinant H3K18ac antibody (RM166, 2 ug/ml) as the detect.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF test of HeLa cells treated with sodium butyrate using recombinant H3K18ac antibody (red). Actin filaments have been labeled with fluorescein phalloidin (green).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC staining of human HepG2 cells using recombinant H3K18ac antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ChIP performed on HeLa cells with or without sodium butyrate treatment using recombinant H3K18ac antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- The recombinant H3K18ac antibody specifically reacts to Histone H3 acetylated at Lysine 18 (K18ac). No cross reactivity with acetylated 4 (K4ac), 9 (K9ac), 14 (K14ac), 23 (K23ac), 27 (K27ac), 36 (K36ac), 56 (K56ac), 79 (K79ac), or 122 (K122) in Histone H3.
