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antibody from NSJ Bioreagents
Targeting: H3-3B
H3.3B, H3F3B
Western blot
ELISA Immunocytochemistry Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- R20220 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant H3K79me2 Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific Recombinant H3K79me2 antibody is suitable for use in Western blot/ELISA/ChImmunoprecipitation/Immunohistochemistry applications with all species samples.
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM181
- Vial size
- 25 ug, 100 ug
- Concentration
- 1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
- Storage
- Store the recombinant H3K79me2 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2) using recombinant H3K79me2 antibody at 0.25 ug/ml showed a band of Histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC staining of human HepG2 cells with recombinant H3K79me2 antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ChIP performed on HeLa cells using the recombinant H3K79me2 antibody (5ug). Real-time PCR was performed using primers specific to the gene indicated.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- The recombinant H3K79me2 antibody specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with unmodified or trimethylated Lysine 79, or other H3 methylations.
