H00003021-M01

antibody from Abnova Corporation

Targeting: H3-3B H3.3B, H3F3B

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003021-M01

Provider

Abnova Corporation

Proper citation

Product name

H3F3B monoclonal antibody (M01), clone 2D7-H1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant H3F3B.

Antigen sequence

MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGG
VKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQR
LVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLF
EDTNLCAIHAKRVTIMPKDIQLARRIRGERA


Isotype

IgG

Antibody clone number

2D7-H1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation.

Pradhan SK, Su T, Yen L, Jacquet K, Huang C, Côté J, Kurdistani SK, Carey MF
Molecular cell 2016 Jan 7;61(1):27-38

---

Chromatin and transcription transitions of mammalian adult germline stem cells and spermatogenesis.

Hammoud SS, Low DH, Yi C, Carrell DT, Guccione E, Cairns BR
Cell stem cell 2014 Aug 7;15(2):239-53

---

Drosophila Yemanuclein and HIRA cooperate for de novo assembly of H3.3-containing nucleosomes in the male pronucleus.

Orsi GA, Algazeery A, Meyer RE, Capri M, Sapey-Triomphe LM, Horard B, Gruffat H, Couble P, Aït-Ahmed O, Loppin B
PLoS genetics 2013;9(2):e1003285

---

Histone variant H3.3 maintains a decondensed chromatin state essential for mouse preimplantation development.

Lin CJ, Conti M, Ramalho-Santos M
Development (Cambridge, England) 2013 Sep;140(17):3624-34

---

HIRA dependent H3.3 deposition is required for transcriptional reprogramming following nuclear transfer to Xenopus oocytes.

Jullien J, Astrand C, Szenker E, Garrett N, Almouzni G, Gurdon JB
Epigenetics & chromatin 2012 Oct 29;5(1):17

---

The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.

Drané P, Ouararhni K, Depaux A, Shuaib M, Hamiche A
Genes & development 2010 Jun 15;24(12):1253-65

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of H3F3B expression in transfected 293T cell line by H3F3B monoclonal antibody (M01), clone 2D7-H1.Lane 1: H3F3B transfected lysate(15 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged H3F3B is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to H3F3B on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to H3F3B on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol