M06819

antibody from Boster Biological Technology

Targeting: H3-3B H3.3B, H3F3B

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M06819

Provider

Boster Biological Technology

Product name

Anti-Histone H3.3 H3F3A Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for H3F3AH3F3B detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat H3F3AH3F3B information: Molecular Weight: 15328 MW; Subcellular Localization: Nucleus. Chromosome.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

EBF-8

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Porcine circovirus type 2 infection inhibits macrophage M1 polarization induced by other pathogens via viral capsid protein and host gC1qR protein.

Yang X, Du Q, Wang X, Shi J, Wang T, Li P, Zhong J, Tong D, Huang Y
Veterinary microbiology 2023 Oct;285:109871

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Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR.

Wang Z, Chen J, Zhang QG, Huang K, Ma D, Du Q, Tong D, Huang Y
Veterinary microbiology 2022 Mar;266:109354

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2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity.

Kusi M, Zand M, Lin LL, Chen M, Lopez A, Lin CL, Wang CM, Lucio ND, Kirma NB, Ruan J, Huang TH, Mitsuya K
Cell reports 2022 Jan 11;38(2):110220

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Porcine circovirus type 2 infection attenuates the K63-linked ubiquitination of STING to inhibit IFN-β induction via p38-MAPK pathway.

Wu X, Wang Z, Qiao D, Yuan Y, Han C, Yang N, Li R, Du Q, Tong D, Huang Y
Veterinary microbiology 2021 Jul;258:109098

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse stomach, using Histone H33 Antibody (M06819)H3F3A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-H3F3A Antibody (M06819)overnight at 4?? Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37?? The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image