Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 11-7007-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-Histone H3.3 Antibody
- Antibody type
- Polyclonal
- Description
- Histones are major chromatin proteins involved in nucleosome assembly. Their major function is to compact long DNA strands inside the nucleus and chromatin regulation. Histone H3.3 is a replication independent variant of Histone H3 which is generally expressed in quiescent or terminally differentiated cells. Modifications in Histone H3.3 are associated with transcriptional activation and acts as an epigenetic mark of transcriptionally active chromatin. Histone H3.3 is a 15 kDa protein which is expressed throughout the cell cycle independently of DNA synthesis in all eukaryotic genomes.
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
Full length recombinant Histone H3.
3 protein was used as the immunogen
for this antibody.- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freezing and thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of Histone H3.3. Anti-Histone H3.3 antibody (11-7007) was used at 2 µg/ml on 1) Raji, 2) Jurkat lysates.
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Histone H3.3. Anti-Histone H3.3 antibody (11-7007) in human Lung tissue at 5 µg/ml.