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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- 703662 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H3.3 G34V oncohistone mutant Recombinant Rabbit Monoclonal Antibody (1H32L36), ChIP-Verified
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 1H32L36
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-H3.3 G34V Rabbit Recombinant Monoclonal Antibody (Product # 703662) and a 17 kDa band corresponding to H3.3 G34V was observed in histone H3.3 G34V overexpressing HEK-293T cells confirming the specificity of H3.3 G34V antibody. Modified whole cell extracts (1% SDS) (30 µg lysate) from control (vector only transfected) (Lane 1), FLAG-tagged histone H3.3 G34V overexpressing HEK-293T cells (Lane 2) and FLAG-tagged histone H3.3 G34R overexpressing HEK-293T cells (Lane 3) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:5000 dilution) and detected by chemiluminescence using Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Antibody specificity was demonstrated by detection of enrichment of the targeted histone modification using SNAP-ChIP™ Spike-in, a proprietary technology developed by EpiCypher™. SNAP-ChIP™ spike-in was performed using H3.3 G34V Recombinant Rabbit Monoclonal Antibody (Product # 703662) and H3.3 G34V was enriched compared to the other histone modifications in the SNAP-ChIP™ OncoStat Panel (Product # A47343).
