M06819-23

antibody from Boster Biological Technology

Targeting: H3-3B H3.3B, H3F3B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

M06819-23

Provider

Boster Biological Technology

Product name

Anti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181

Antibody type

Monoclonal

Description

Anti-Dimethyl Histone H3 (Lys79) Rabbit Monoclonal Antibody, Clone#RM181 tested in WB, ELISA, Multiplex, ChIP, IHC, reactive to All Vertebrates

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

RM181

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

-20°C

References

Submitted references

2-Hydroxyglutarate destabilizes chromatin regulatory landscape and lineage fidelity to promote cellular heterogeneity.

Kusi M, Zand M, Lin LL, Chen M, Lopez A, Lin CL, Wang CM, Lucio ND, Kirma NB, Ruan J, Huang TH, Mitsuya K
Cell reports 2022 Jan 11;38(2):110220

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western Blotting resultWestern Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.

Additional image