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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
- Other assay [2]
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- Product number
- PA5-29350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDE Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, H1299, HeLa, HepG2, mouse brain, rat brain. Predicted reactivity: Mouse (94%), Rat (95%), Zebrafish (90%), Dog (93%), Rhesus Monkey (99%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.6 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Abelmoschus esculentus subfractions attenuate Aβ and tau by regulating DPP-4 and insulin resistance signals.
Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease.
Huang CN, Wang CJ, Lin CL, Li HH, Yen AT, Peng CH
BMC complementary medicine and therapies 2020 Dec 2;20(1):370
BMC complementary medicine and therapies 2020 Dec 2;20(1):370
Simvastatin inhibits the apoptosis of hippocampal cells in a mouse model of Alzheimer's disease.
Hu X, Song C, Fang M, Li C
Experimental and therapeutic medicine 2018 Feb;15(2):1795-1802
Experimental and therapeutic medicine 2018 Feb;15(2):1795-1802
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of insulin degrading enzyme showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using an Insulin degrading enzyme polyclonal antibody (Product # PA5-29350) diluted at 1:500. Blue: Hoechst 33343 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDE Polyclonal Antibody detects IDE protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IDE stained by IDE Polyclonal Antibody (Product # PA5-29350) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 7 Effect of AE on attenuating p-Tau. SK-N-MC cells were incubated for 24 h with or without 50 muM of palmitate, and with different concentrations of F1 ( a ) and F2 ( b ). The expressions of IDE and p-Tau were analyzed by Western blotting. Data are presented as means +- SD ( n = 3) and analysed with ANOVA. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with the control. # p < 0.05, # p < 0.01, # p < 0.001 compared with the palmitate-treated only
