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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA1-16809 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Apolipoprotein A5 Monoclonal Antibody (1G5G9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- The target sequence has 83% sequence homology with bovine, 79% sequence homology with rat and 78% sequence homology with mouse.
- Reactivity
- Human, Hamster
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1G5G9
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references A common variant in 11q23.3 associated with hyperlipidemia is mediated by the binding and regulation of GATA4.
Chou WC, Chen WT, Shen CY
NPJ genomic medicine 2022 Jan 19;7(1):4
NPJ genomic medicine 2022 Jan 19;7(1):4
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Apolipoprotein A5 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Apolipoprotein A5 (1G5G9) Mouse Monoclonal Antibody (Product # MA1-16809) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Apolipoprotein A5 in 1 x 10^6 CHO (A) and HEK-293 (B) cells. Samples were incubated in Apolipoprotein A5 monoclonal antibody (Product # MA1-16809) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.
