710262

antibody from Invitrogen Antibodies

Targeting: APOC3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

710262

Provider

Invitrogen Antibodies

Product name

ApoC3 Recombinant Superclonal™ Antibody (6HCLC)

Antibody type

Other

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse, non-human primate, rabbit and porcine based on sequence homology. Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

6HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Hypercholesterolemia-Induced HDL Dysfunction Can Be Reversed: The Impact of Diet and Statin Treatment in a Preclinical Animal Model.

Schoch L, Sutelman P, Suades R, Casani L, Padro T, Badimon L, Vilahur G
International journal of molecular sciences 2022 Aug 2;23(15)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ApoC3 in HepG2 cells using an ApoC3 Recombinant Rabbit Polyclonal Antibody (Product # 710262) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing subcellular cytoplasmic localization of Apo C3.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ApoC3 in HepG2 cells using an ApoC3 Recombinant Rabbit Superclonal™ Antibody (Product # 710262) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing subcellular cytoplasmic localization of Apo C3.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Effects of diet and rosuvastatin intervention on HDL apolipoprotein levels. Changes in protein levels of apolipoproteins (APO) APOA-I ( A ), APOC-III ( B ), and APOM ( C ) in isolated HDL particles are presented as relative quantification and representative Western blots ( D ) at study endpoint. Western blot quantifications are shown as mean +- SD. Shapiro-Wilk test confirmed normality (alpha = 0.05), and data were analyzed by unpaired t-test and considered significant with a p -value < 0.05. ** p < 0.0001 in comparison to day 0; ++ p < 0.0001 in comparison to day 10; # p < 0.05 in comparison to day 40 of animals on HC diet. d0-40: day 0-40; HC: hypercholesterolemic diet; NC: normocholesterolemic diet; +R: + rosuvastatin.