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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 710262 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ApoC3 Recombinant Polyclonal Antibody (6HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse, non-human primate, rabbit and porcine based on sequence homology.
- Antibody clone number
- 6HCLC
- Concentration
- 0.5 mg/mL
Submitted references Hypercholesterolemia-Induced HDL Dysfunction Can Be Reversed: The Impact of Diet and Statin Treatment in a Preclinical Animal Model.
Schoch L, Sutelman P, Suades R, Casani L, Padro T, Badimon L, Vilahur G
International journal of molecular sciences 2022 Aug 2;23(15)
International journal of molecular sciences 2022 Aug 2;23(15)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on 2 µL plasma (Lane 1) and 3 µL plasma (Lane 2) from a 50 year old male Alzheimer's patient. The blots were probed with Anti-Apolipoprotein C-3 Recombinant Rabbit Polyclonal Antibody (Product # 710262, 1-3 µg/mL) and detected by chemiluminescence Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 28 kDa band corresponding to Apolipoprotein C-3 was observed across the plasma samples tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ApoC3 in whole cell extracts from rat liver lysate using an ApoC3 Recombinant Rabbit Polyclonal Antibody (Product # 710262) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~13kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ApoC3 in HepG2 cells using an ApoC3 Recombinant Rabbit Polyclonal Antibody (Product # 710262) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing subcellular cytoplasmic localization of Apo C3.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Effects of diet and rosuvastatin intervention on HDL apolipoprotein levels. Changes in protein levels of apolipoproteins (APO) APOA-I ( A ), APOC-III ( B ), and APOM ( C ) in isolated HDL particles are presented as relative quantification and representative Western blots ( D ) at study endpoint. Western blot quantifications are shown as mean +- SD. Shapiro-Wilk test confirmed normality (alpha = 0.05), and data were analyzed by unpaired t-test and considered significant with a p -value < 0.05. ** p < 0.0001 in comparison to day 0; ++ p < 0.0001 in comparison to day 10; # p < 0.05 in comparison to day 40 of animals on HC diet. d0-40: day 0-40; HC: hypercholesterolemic diet; NC: normocholesterolemic diet; +R: + rosuvastatin.
