Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-78802 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ApoC3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 500 µg/mL
- Storage
- -20°C
Submitted references Hepatocyte Small Heterodimer Partner Mediates Sex-Specific Effects on Triglyceride Metabolism via Androgen Receptor in Male Mice.
Palmisano BT, Zhu L, Litts B, Burman A, Yu S, Neuman JC, Anozie U, Luu TN, Edington EM, Stafford JM
Metabolites 2021 May 20;11(5)
Metabolites 2021 May 20;11(5)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of ApoC3 in SiHa cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with ApoC3 Polyclonal Antibody (Product # PA5-78802) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry of ApoC3 in U2OS cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with ApoC3 Polyclonal Antibody (Product # PA5-78802) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Effect of hepatic deletion of SHP on lipoprotein lipase activities. ( A - F ) Plasma ApoC1, ApoC2, ApoC3, ApoE, and ApoA1 levels were determined with immunoblotting and blot quantifications. ( G ) Liver mRNA levels of AR in intact or GDX fl/fl and SHP Deltahep mice. ( H , I ) Plasma from chow ( H ) and fructose ( I ) fed mice was used to evaluate lipoprotein lipase activity. Data shown are mean +- SD ( n = 6~8/group), # p < 0.05 and ## p < 0.01 for genotype effect, and $$ p < 0.01 and $$$ p < 0.001 for treatment effects (2-way ANOVA). * p < 0.05 ( t -test).