Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-22798 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NOTCH4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human healthy CD4 T cells were islolated and stimulated with CD3/CD28 microbeads for 72 hours. Two lanes are biological replicates. Western blot analysis of NOTCH4 was performed by loading whole-cell lysates of activated human CD4 T cells. NOTCH4 was detected at approximately 210 kDa, 60kDa and 40kDa using a NOTCH4 polyclonal Antibody (Product # PA5-22798) at a dilution of 1:1000 in Blocking Buffer overnight at 4°C on a rocking platform, followed by a 1-hour room-temperature incubation of a goat anti-rabbit IgG antibody at a dilution of 1:5000. Data courtesy of Thermo Scientific KOL Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NOTCH4 in Jurkat, U937, WEHI and Raw267 cell lysates. Sample was incubated in NOTCH4 polyclonal antibody (Product # PA5-22798).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of NOTCH4 in human intestine. Samples were incubated in NOTCH4 polyclonal antibody (Product # PA5-22798).