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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX103429 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103429, RRID:AB_10729206
- Product name
- TIE1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIE1 antibody detects TIE1 protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of human TIE1-transfected 293T cells5 % SDS-PAGETIE1 antibody (GTX103429) dilution: 1:5000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIE1 antibody detects TIE1 protein by Western blot analysis.A. 30 µg BCL-1 whole cell lysate/extract7.5 % SDS-PAGETIE1 antibody (GTX103429) dilution: 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TIE1 antibody detects TIE1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with TIE1 antibody (GTX103429) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TIE1(GTX103429) antibody at 1:500 dilution.
