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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- NBP1-18610 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-18610, RRID:AB_1625507
- Product name
- Mouse Monoclonal Tie-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The monoclonal antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Tie-1 Antibody (Cl. 6F12) [NBP1-18610] - Analysis of recombinant human and mouse soluble TIE receptors using a monoclonal antibody directed against human recombinant soluble TIE-1. There is a weak cross reactivity with human sTIE-2 but no reactivity with mouse sTIE-1 and sTIE-2 visible.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Tie-1 Antibody (Cl. 6F12) [NBP1-18610] - Analysis using the Biotin conjugate of NBP1-18610. Staining of primary human dermal microvascular endothelial cells (HDMVEC). Upper panel: no primary antibody; Middle panel: solely conjugated secondary antibody; Lower panel: Biotin-conjugated anti-human TIE-1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Tie-1 Antibody (Cl. 6F12) [NBP1-18610] - Fig. 1: Flow analysis of TIE-1 expression in primary human umbilical vein endothelial cells (HUVEC).
