AF619

antibody from R&D Systems

Targeting: TIE1 JTK14, TIE

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF619

Provider

R&D Systems

Product name

Human Tie-1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Tie-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Tie-1 is observed and less than 1% cross-reactivity with recombinant human Tie-2 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P35590


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Structural basis of Tie2 activation and Tie2/Tie1 heterodimerization.

Leppänen VM, Saharinen P, Alitalo K
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 25;114(17):4376-4381

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Polydom Is an Extracellular Matrix Protein Involved in Lymphatic Vessel Remodeling.

Morooka N, Futaki S, Sato-Nishiuchi R, Nishino M, Totani Y, Shimono C, Nakano I, Nakajima H, Mochizuki N, Sekiguchi K
Circulation research 2017 Apr 14;120(8):1276-1288

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Endothelial destabilization by angiopoietin-2 via integrin β1 activation.

Hakanpaa L, Sipila T, Leppanen VM, Gautam P, Nurmi H, Jacquemet G, Eklund L, Ivaska J, Alitalo K, Saharinen P
Nature communications 2015 Jan 30;6:5962

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Regulated proteolytic processing of Tie1 modulates ligand responsiveness of the receptor-tyrosine kinase Tie2.

Marron MB, Singh H, Tahir TA, Kavumkal J, Kim HZ, Koh GY, Brindle NP
The Journal of biological chemistry 2007 Oct 19;282(42):30509-17

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Interaction between Tie receptors modulates angiogenic activity of angiopoietin2 in endothelial progenitor cells.

Kim KL, Shin IS, Kim JM, Choi JH, Byun J, Jeon ES, Suh W, Kim DK
Cardiovascular research 2006 Dec 1;72(3):394-402

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Expansion of human SCID-repopulating cells under hypoxic conditions.

Danet GH, Pan Y, Luongo JL, Bonnet DA, Simon MC
The Journal of clinical investigation 2003 Jul;112(1):126-35

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Evidence for heterotypic interaction between the receptor tyrosine kinases TIE-1 and TIE-2.

Marron MB, Hughes DP, Edge MD, Forder CL, Brindle NP
The Journal of biological chemistry 2000 Dec 15;275(50):39741-6

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Tie-1 in Human Placenta. Tie-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 8 µg/mL Goat Anti-Human Tie-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Tie-1 in HUVEC Human Cells by Flow Cytometry. HUVEC human umbilical vein endothelial cells were stained with Goat Anti-Human Tie-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF619, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).