PA5-19784

antibody from Invitrogen Antibodies

Targeting: APOA1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

PA5-19784

Provider

Invitrogen Antibodies

Product name

ApoA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with dog, pig and baboon based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.4 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Inhibition of apolipoprotein B synthesis stimulates endoplasmic reticulum autophagy that prevents steatosis.

Conlon DM, Thomas T, Fedotova T, Hernandez-Ono A, Di Paolo G, Chan RB, Ruggles K, Gibeley S, Liu J, Ginsberg HN
The Journal of clinical investigation 2016 Oct 3;126(10):3852-3867

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Human Lymph node Tissue Lysate using Product # PA5-19784, Apolipoprotein A I primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Human Testis Tissue Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-ApoA1 Polyclonal Antibody(Product # PA5-19784) and a 25kDa band corresponding to ApoA1 was observed across cell lines tested. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), Hep G2 treated with FLI, 10 uM, 4 Hrs. (Lane 2), Hep G2 treated with PTI, 1X, 4Hrs. (Lane 3), SH-SY5Y (Lane 4), SH-SY5Y treated with FLI, 10 uM, 4 Hrs (Lane 5), SH-SY5Y treated with PTI, 1X, 4Hrs. (Lane 6), Empty lane (Lane 7) and Human Plasma (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Expression of APOA1 was found to be higher in Hep G2 cell line as compared to SH-SY 5Y.