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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-21528 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MRPS5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (80%), Rat (81%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MRPS5 using 30 µg of A) A431 (B) H1299 (C) HeLa S3 and D) HepG2 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a MRPS5 polyclonal antibody (Product # PA5-21528) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of MRPS5 was performed by separating 50 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a MRPS5 Polyclonal Antibody (Product # PA5-21528) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MRPS5 in methanol-fixed HeLa cells using a MRPS5 polyclonal antibody (Product # PA5-21528) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using MRPS5 (Product # PA5-21528) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MRPS5 was performed in paraffin-embedded mouse kidney tissue using MRPS5 Polyclonal Antibody (Product # PA5-21528) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MRPS5 Polyclonal Antibody detects MRPS5 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. MRPS5 Polyclonal Antibody (Product # PA5-21528) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
