PA5-120484

antibody from Invitrogen Antibodies

Targeting: ABCA2 ABC2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

PA5-120484

Provider

Invitrogen Antibodies

Product name

ABCA2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: HeLa, Rat brain. The target is usually found in the following locations: Endosome membrane, Lysosome membrane, Multi-pass membrane protein. Immunogen sequence: SHLDRSTVSS FSLDSVARNP QELWRFLTQN LSLPNSTAQA LLAARVDPPE VYHLLFGPSS ALDSQSGLHK GQEPWSRLGG NPLFRMEELL LAPALLEQLT CTPGSGELGR ILTVPESQKG ALQGYRDAVC SGQAAARARR FSGLSAELRN QLDVAKVSQQ LGLDAPNGSD SSPQAPPPRR LQALLGDLLD AQKVLQDVDV LSALALLLPQ G

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.7 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ABCA2 in various cell lines using a ABCA2 Polyclonal antibody (Product # PA5-120484) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3 min.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ABCA2 in mouse brain using a ABCA2 Polyclonal antibody (Product # PA5-120484). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of ABCA2 in mouse brain using a ABCA2 Polyclonal antibody (Product # PA5-120484). Blue: DAPI for nuclear staining.