Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28963 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AKR1D1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: A549. Predicted reactivity: Mouse (82%), Rabbit (89%), Chicken (81%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Silencing of the transcriptional factor ZEB1 alters the steroidogenic pathway, and increases the concentration of testosterone and DHT in DU145 cells.
Androgenic potential of human fetal adrenals at the end of the first trimester.
Herrera D, Orellana-Serradell O, Villar P, Torres MJ, Paciucci R, Castellón EA, Contreras HR
Oncology reports 2019 Feb;41(2):1275-1283
Oncology reports 2019 Feb;41(2):1275-1283
Androgenic potential of human fetal adrenals at the end of the first trimester.
Savchuk I, Morvan ML, Antignac JP, Gemzell-Danielsson K, Le Bizec B, Söder O, Svechnikov K
Endocrine connections 2017 Aug;6(6):348-359
Endocrine connections 2017 Aug;6(6):348-359
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AKR1D1 Polyclonal Antibody (Product # PA5-28963). Sample (30 µg of whole cell lysate). Lane A: A549. 10% SDS PAGE. AKR1D1 Polyclonal Antibody (Product # PA5-28963) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using AKR1D1 (Product # PA5-28963) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.