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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F54747 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Trim24 Antibody / Tif1 alpha / Tif1a
- Antibody type
- Polyclonal
- Description
- This highly specific Trim24 antibody is suitable for use in Western blot/Immunofluorescence/Immunohistochemistry applications with mouse samples.
- Reactivity
- Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the Trim24 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of mouse 1) NIH 3T3, 2) spleen, 3) testis and 4) thymus lysate with Trim 24 antibody. Expected molecular weight: 117-140 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human HeLa cells with Trim24 antibody (green) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse testis tissue with Trim24 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
