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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P162BT-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CLEC11A Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: GHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ AAAQARCTAR GGSLAQPADR QQMEALTRYL RAALAPYNWP VWLGVHDRRA EGLYLFENGQ RVSFFAWHRS PRPELGAQPS ASPHPLSPDQ PNGGTLENCV AQASDDGSWW DHDCQRRLYY VCEFPF. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human SCGF-alpha. Anti-Human SCGF-alpha-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human SCGF-alpha by sandwich ELISA (using 100 µL/well) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human SCGF-alpha (500-P162) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human SCGF-alpha. Western Blot: To detect Human SCGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human SCGF-alpha is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions. 500-P162BT-1 mg will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 2 x 500 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human SCGF-alpha by Western Blot analysis CLEC11A Polyclonal Antibody, Biotin (Product # 500-P162BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human SCGF-alpha is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human SCGF-alpha by Western Blot analysis CLEC11A Polyclonal Antibody, Biotin (Product # 500-P162BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human SCGF-alpha is 1.5-3.0 ng/lane, under either reducingor non-reducing conditions.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human SCGF-alpha by sandwich ELISA (using 100 µl/well) a concentration of 0.25-1.0 µg/mL of CLEC11A Polyclonal Antibody, Biotin (Product # 500-P162BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech CLEC11A Polyclonal Antibody (Product # 500-P162-1MG) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human SCGF-alpha.
- Conjugate
- Biotin
