Antibody data
- Antibody Data
- Antigen structure
- References [26]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- 22227-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#22227-1-AP, RRID:AB_2651175
- Product name
- CEP164 antibody
- Antibody type
- Polyclonal
- Description
- CEP164 antibody (Cat. #22227-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, Canine and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Canine
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Analyzing Centrioles and Cilia by Expansion Microscopy.
The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.
NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.
TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity.
OCRL regulates lysosome positioning and mTORC1 activity through SSX2IP-mediated microtubule anchoring.
Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development.
ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.
Exocyst complex mediates recycling of internal cilia.
WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.
Prolonged mitosis results in structurally aberrant and over-elongated centrioles.
Ciliogenesis and Hedgehog signalling are suppressed downstream of KRAS during acinar-ductal metaplasia in mouse.
NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling.
The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.
Cep128 associates with Odf2 to form the subdistal appendage of the centriole.
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.
Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.
Expansion microscopy for the analysis of centrioles and cilia.
M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.
Oncoprotein CIP2A promotes the disassembly of primary cilia and inhibits glycolytic metabolism.
Glucose deprivation induces primary cilium formation through mTORC1 inactivation.
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.
The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.
Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis.
DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.
Genetic link between renal birth defects and congenital heart disease.
TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).
Kong D, Loncarek J
Methods in molecular biology (Clifton, N.J.) 2021;2329:249-263
Methods in molecular biology (Clifton, N.J.) 2021;2329:249-263
The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.
Stemm-Wolf AJ, O'Toole ET, Sheridan RM, Morgan JT, Pearson CG
Molecular biology of the cell 2021 Oct 1;32(20):ar4
Molecular biology of the cell 2021 Oct 1;32(20):ar4
NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.
Liu M, Zhang W, Li M, Feng J, Kuang W, Chen X, Yang F, Sun Q, Xu Z, Hua J, Yang C, Liu W, Shu Q, Yang Y, Zhou T, Xie S
Cell research 2021 Nov;31(11):1199-1211
Cell research 2021 Nov;31(11):1199-1211
TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity.
Meitinger F, Kong D, Ohta M, Desai A, Oegema K, Loncarek J
The Journal of cell biology 2021 Jul 5;220(7)
The Journal of cell biology 2021 Jul 5;220(7)
OCRL regulates lysosome positioning and mTORC1 activity through SSX2IP-mediated microtubule anchoring.
Wang B, He W, Prosseda PP, Li L, Kowal TJ, Alvarado JA, Wang Q, Hu Y, Sun Y
EMBO reports 2021 Jul 5;22(7):e52173
EMBO reports 2021 Jul 5;22(7):e52173
Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development.
Xiao C, Grzonka M, Meyer-Gerards C, Mack M, Figge R, Bazzi H
EMBO reports 2021 Feb 3;22(2):e51127
EMBO reports 2021 Feb 3;22(2):e51127
ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.
Evans LT, Anglen T, Scott P, Lukasik K, Loncarek J, Holland AJ
The EMBO journal 2021 Feb 15;40(4):e105106
The EMBO journal 2021 Feb 15;40(4):e105106
Exocyst complex mediates recycling of internal cilia.
Rivera-Molina FE, Xi Z, Reales E, Wang B, Toomre D
Current biology : CB 2021 Dec 20;31(24):5580-5589.e5
Current biology : CB 2021 Dec 20;31(24):5580-5589.e5
WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.
Steib E, Laporte MH, Gambarotto D, Olieric N, Zheng C, Borgers S, Olieric V, Le Guennec M, Koll F, Tassin AM, Steinmetz MO, Guichard P, Hamel V
eLife 2020 Sep 18;9
eLife 2020 Sep 18;9
Prolonged mitosis results in structurally aberrant and over-elongated centrioles.
Kong D, Sahabandu N, Sullenberger C, Vásquez-Limeta A, Luvsanjav D, Lukasik K, Loncarek J
The Journal of cell biology 2020 Jun 1;219(6)
The Journal of cell biology 2020 Jun 1;219(6)
Ciliogenesis and Hedgehog signalling are suppressed downstream of KRAS during acinar-ductal metaplasia in mouse.
Bangs FK, Miller P, O'Neill E
Disease models & mechanisms 2020 Jul 30;13(7)
Disease models & mechanisms 2020 Jul 30;13(7)
NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling.
Martin-Hurtado A, Martin-Morales R, Robledinos-Antón N, Blanco R, Palacios-Blanco I, Lastres-Becker I, Cuadrado A, Garcia-Gonzalo FR
Scientific reports 2019 Sep 25;9(1):13896
Scientific reports 2019 Sep 25;9(1):13896
The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.
Cuenca A, Insinna C, Zhao H, John P, Weiss MA, Lu Q, Walia V, Specht S, Manivannan S, Stauffer J, Peden AA, Westlake CJ
The Journal of biological chemistry 2019 Oct 18;294(42):15418-15434
The Journal of biological chemistry 2019 Oct 18;294(42):15418-15434
Cep128 associates with Odf2 to form the subdistal appendage of the centriole.
Kashihara H, Chiba S, Kanno SI, Suzuki K, Yano T, Tsukita S
Genes to cells : devoted to molecular & cellular mechanisms 2019 Mar;24(3):231-243
Genes to cells : devoted to molecular & cellular mechanisms 2019 Mar;24(3):231-243
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.
Bowler M, Kong D, Sun S, Nanjundappa R, Evans L, Farmer V, Holland A, Mahjoub MR, Sui H, Loncarek J
Nature communications 2019 Mar 1;10(1):993
Nature communications 2019 Mar 1;10(1):993
Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.
Odabasi E, Gul S, Kavakli IH, Firat-Karalar EN
EMBO reports 2019 Jun;20(6)
EMBO reports 2019 Jun;20(6)
Expansion microscopy for the analysis of centrioles and cilia.
Sahabandu N, Kong D, Magidson V, Nanjundappa R, Sullenberger C, Mahjoub MR, Loncarek J
Journal of microscopy 2019 Dec;276(3):145-159
Journal of microscopy 2019 Dec;276(3):145-159
M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.
Huang N, Zhang D, Li F, Chai P, Wang S, Teng J, Chen J
Nature communications 2018 Oct 30;9(1):4511
Nature communications 2018 Oct 30;9(1):4511
Oncoprotein CIP2A promotes the disassembly of primary cilia and inhibits glycolytic metabolism.
Jeong AL, Ka HI, Han S, Lee S, Lee EW, Soh SJ, Joo HJ, Sumiyasuren B, Park JY, Lim JS, Park JH, Lee MS, Yang Y
EMBO reports 2018 May;19(5)
EMBO reports 2018 May;19(5)
Glucose deprivation induces primary cilium formation through mTORC1 inactivation.
Takahashi K, Nagai T, Chiba S, Nakayama K, Mizuno K
Journal of cell science 2018 Jan 8;131(1)
Journal of cell science 2018 Jan 8;131(1)
VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.
Ott C, Nachmias D, Adar S, Jarnik M, Sherman S, Birnbaum RY, Lippincott-Schwartz J, Elia N
Scientific reports 2018 Feb 20;8(1):3353
Scientific reports 2018 Feb 20;8(1):3353
The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.
Kanie T, Abbott KL, Mooney NA, Plowey ED, Demeter J, Jackson PK
Developmental cell 2017 Jul 10;42(1):22-36.e12
Developmental cell 2017 Jul 10;42(1):22-36.e12
Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis.
Hamel V, Steib E, Hamelin R, Armand F, Borgers S, Flückiger I, Busso C, Olieric N, Sorzano COS, Steinmetz MO, Guichard P, Gönczy P
Current biology : CB 2017 Aug 21;27(16):2486-2498.e6
Current biology : CB 2017 Aug 21;27(16):2486-2498.e6
DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.
Xu X, Huang S, Zhang B, Huang F, Chi W, Fu J, Wang G, Li S, Jiang Q, Zhang C
Nature communications 2017 Apr 27;8:15164
Nature communications 2017 Apr 27;8:15164
Genetic link between renal birth defects and congenital heart disease.
San Agustin JT, Klena N, Granath K, Panigrahy A, Stewart E, Devine W, Strittmatter L, Jonassen JA, Liu X, Lo CW, Pazour GJ
Nature communications 2016 Mar 22;7:11103
Nature communications 2016 Mar 22;7:11103
TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).
Stephen LA, Tawamie H, Davis GM, Tebbe L, Nürnberg P, Nürnberg G, Thiele H, Thoenes M, Boltshauser E, Uebe S, Rompel O, Reis A, Ekici AB, McTeir L, Fraser AM, Hall EA, Mill P, Daudet N, Cross C, Wolfrum U, Jamra RA, Davey MG, Bolz HJ
eLife 2015 Sep 19;4
eLife 2015 Sep 19;4
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CEP164 antibody 22227-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
- Other comments
- IF
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- WB results of CEP164 (22227-1-AP, 1:500) with RPE1 cells by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.
- Other comments
- IP