22227-1-AP

antibody from Proteintech Group

Targeting: CEP164 KIAA1052, NPHP15

Provider product page for 22227-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [56]
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Validations
Immunocytochemistry [1]
Immunoprecipitation [1]

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Product number: 22227-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#22227-1-AP, RRID:AB_2651175
Product name: CEP164 antibody
Antibody type: Polyclonal
Description: CEP164 antibody (Cat. #22227-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, Canine and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Canine
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CEP164 protein structure - 22227-1-AP shown in red.

Submitted references Immunolabel-First-Expand-Later Expansion Microscopy Approach Using Stable STED Dyes.

Kong D, Luvsanjav D, Loncarek J
Methods in molecular biology (Clifton, N.J.) 2024;2725:89-101

Differential subcellular localization of ASPM RNA and protein.

Lo HG, Pearson CG, Taliaferro JM
microPublication biology 2024;2024

XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis.

Hong R, Tan Y, Tian X, Huang Z, Wang J, Ni H, Yang J, Bu W, Yang S, Li T, Yu F, Zhong W, Sun T, Wang X, Li D, Liu M, Yang Y, Zhou J
EMBO reports 2024 Mar;25(3):1055-1074

Unraveling cellular complexity with transient adapters in highly multiplexed super-resolution imaging.

Schueder F, Rivera-Molina F, Su M, Marin Z, Kidd P, Rothman JE, Toomre D, Bewersdorf J
Cell 2024 Mar 28;187(7):1769-1784.e18

The ARPKD Protein DZIP1L Regulates Ciliary Protein Entry by Modulating the Architecture and Function of Ciliary Transition Fibers.

Chen H, Wu Z, Yan Z, Chen C, Zhang Y, Wang Q, Gao Y, Ling K, Hu J, Wei Q
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2024 Jun;11(24):e2308820

Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells.

Grzonka M, Bazzi H
eLife 2024 Feb 26;13

Prolonged overexpression of PLK4 leads to formation of centriole rosette clusters that are connected via canonical centrosome linker proteins.

Ozcan SC, Kalkan BM, Cicek E, Canbaz AA, Acilan C
Scientific reports 2024 Feb 22;14(1):4370

Time-series reconstruction of the molecular architecture of human centriole assembly.

Laporte MH, Gambarotto D, Bertiaux É, Bournonville L, Louvel V, Nunes JM, Borgers S, Hamel V, Guichard P
Cell 2024 Apr 25;187(9):2158-2174.e19

Expansion Microscopy of Mouse Photoreceptor Cilia.

Moye AR, Robichaux MA, Wensel T
Advances in experimental medicine and biology 2023;1415:395-402

Brain microvascular endothelial cells possess a second cilium that arises from the daughter centriole.

Thirugnanam K, Gupta A, Nunez F, Prabhudesai S, Pan AY, Nauli SM, Ramchandran R
Frontiers in molecular biosciences 2023;10:1250016

INPP5E regulates CD3ζ enrichment at the immune synapse by phosphoinositide distribution control.

Chiu TY, Lo CH, Lin YH, Lai YD, Lin SS, Fang YT, Huang WS, Huang SY, Tsai PY, Yang FH, Chong WM, Wu YC, Tsai HC, Liu YW, Hsu CL, Liao JC, Wang WJ
Communications biology 2023 Sep 5;6(1):911

The IFT81-IFT74 complex acts as an unconventional RabL2 GTPase-activating protein during intraflagellar transport.

Boegholm N, Petriman NA, Loureiro-López M, Wang J, Vela MIS, Liu B, Kanie T, Ng R, Jackson PK, Andersen JS, Lorentzen E
The EMBO journal 2023 Sep 18;42(18):e111807

Urban dust particles disrupt mitotic progression by dysregulating Aurora kinase B-related functions.

Ryu J, Lee SH, Kim S, Jeong JW, Kim KS, Nam S, Kim JE
Journal of hazardous materials 2023 Oct 5;459:132238

Proteomic profiling of centrosomes across multiple mammalian cell and tissue types by an affinity capture method.

Carden S, Vitiello E, Rosa E Silva I, Holder J, Quarantotti V, Kishore K, Roamio Franklin VN, D'Santos C, Ochi T, van Breugel M, Gergely F
Developmental cell 2023 Nov 6;58(21):2393-2410.e9

Ependymal polarity defects coupled with disorganized ciliary beating drive abnormal cerebrospinal fluid flow and spine curvature in zebrafish.

Xie H, Kang Y, Liu J, Huang M, Dai Z, Shi J, Wang S, Li L, Li Y, Zheng P, Sun Y, Han Q, Zhang J, Zhu Z, Xu L, Yelick PC, Cao M, Zhao C
PLoS biology 2023 Mar;21(3):e3002008

A direct interaction between CENTLEIN and RABIN8 is required for primary cilium formation.

Li L, Li J, Yuan L
Acta biochimica et biophysica Sinica 2023 Jul 20;55(9):1434-1444

SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming.

Wu CT, Lidsky PV, Xiao Y, Cheng R, Lee IT, Nakayama T, Jiang S, He W, Demeter J, Knight MG, Turn RE, Rojas-Hernandez LS, Ye C, Chiem K, Shon J, Martinez-Sobrido L, Bertozzi CR, Nolan GP, Nayak JV, Milla C, Andino R, Jackson PK
Cell 2023 Jan 5;186(1):112-130.e20

The evolutionary conserved proteins CEP90, FOPNL, and OFD1 recruit centriolar distal appendage proteins to initiate their assembly.

Le Borgne P, Greibill L, Laporte MH, Lemullois M, Bouhouche K, Temagoult M, Rosnet O, Le Guennec M, Lignières L, Chevreux G, Koll F, Hamel V, Guichard P, Tassin AM
PLoS biology 2022 Sep;20(9):e3001782

Multiple ciliary localization signals control INPP5E ciliary targeting.

Cilleros-Rodriguez D, Martin-Morales R, Barbeito P, Deb Roy A, Loukil A, Sierra-Rodero B, Herranz G, Pampliega O, Redrejo-Rodriguez M, Goetz SC, Izquierdo M, Inoue T, Garcia-Gonzalo FR
eLife 2022 Sep 5;11

Human SFI1 and Centrin form a complex critical for centriole architecture and ciliogenesis.

Laporte MH, Bouhlel IB, Bertiaux E, Morrison CG, Giroud A, Borgers S, Azimzadeh J, Bornens M, Guichard P, Paoletti A, Hamel V
The EMBO journal 2022 Nov 2;41(21):e112107

The mechanism of acentrosomal spindle assembly in human oocytes.

Wu T, Dong J, Fu J, Kuang Y, Chen B, Gu H, Luo Y, Gu R, Zhang M, Li W, Dong X, Sun X, Sang Q, Wang L
Science (New York, N.Y.) 2022 Nov 18;378(6621):eabq7361

ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.

Song T, Yang Y, Zhou P, Ran J, Zhang L, Wu X, Xie W, Zhong T, Liu H, Liu M, Li D, Zhao H, Zhou J
EMBO reports 2022 May 4;23(5):e54090

Evolutionary conservation of centriole rotational asymmetry in the human centrosome.

Gaudin N, Martin Gil P, Boumendjel M, Ershov D, Pioche-Durieu C, Bouix M, Delobelle Q, Maniscalco L, Phan TBN, Heyer V, Reina-San-Martin B, Azimzadeh J
eLife 2022 Mar 23;11

Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly.

Huang M, Kong X, Tang Z, Lin Z, He R, Cao M, Zhang X
Journal of cellular physiology 2022 Jun;237(6):2703-2712

Terminally differentiated osteoclasts organize centrosomes into large clusters for microtubule nucleation and bone resorption.

Philip R, Fiorino C, Harrison RE
Molecular biology of the cell 2022 Jul 1;33(8):ar68

CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesis.

Lee M, Nagashima K, Yoon J, Sun J, Wang Z, Carpenter C, Lee HK, Hwang YS, Westlake CJ, Daar IO
The Journal of cell biology 2022 Jan 3;221(1)

Arih2 regulates Hedgehog signaling through smoothened ubiquitylation and ER-associated degradation.

Lv B, Zhang XO, Pazour GJ
Journal of cell science 2022 Aug 15;135(16)

Analyzing Centrioles and Cilia by Expansion Microscopy.

Kong D, Loncarek J
Methods in molecular biology (Clifton, N.J.) 2021;2329:249-263

The SON RNA splicing factor is required for intracellular trafficking structures that promote centriole assembly and ciliogenesis.

Stemm-Wolf AJ, O'Toole ET, Sheridan RM, Morgan JT, Pearson CG
Molecular biology of the cell 2021 Oct 1;32(20):ar4

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Liu M, Zhang W, Li M, Feng J, Kuang W, Chen X, Yang F, Sun Q, Xu Z, Hua J, Yang C, Liu W, Shu Q, Yang Y, Zhou T, Xie S
Cell research 2021 Nov;31(11):1199-1211

Disrupted intraflagellar transport due to IFT74 variants causes Joubert syndrome.

Luo M, Lin Z, Zhu T, Jin M, Meng D, He R, Cao Z, Shen Y, Lu C, Cai R, Zhao Y, Wang X, Li H, Wu S, Zou X, Luo G, Cao L, Huang M, Jiao H, Gao H, Sui R, Zhao C, Ma X, Cao M
Genetics in medicine : official journal of the American College of Medical Genetics 2021 Jun;23(6):1041-1049

TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity.

Meitinger F, Kong D, Ohta M, Desai A, Oegema K, Loncarek J
The Journal of cell biology 2021 Jul 5;220(7)

OCRL regulates lysosome positioning and mTORC1 activity through SSX2IP-mediated microtubule anchoring.

Wang B, He W, Prosseda PP, Li L, Kowal TJ, Alvarado JA, Wang Q, Hu Y, Sun Y
EMBO reports 2021 Jul 5;22(7):e52173

Rab34 is necessary for early stages of intracellular ciliogenesis.

Stuck MW, Chong WM, Liao JC, Pazour GJ
Current biology : CB 2021 Jul 12;31(13):2887-2894.e4

Gradual centriole maturation associates with the mitotic surveillance pathway in mouse development.

Xiao C, Grzonka M, Meyer-Gerards C, Mack M, Figge R, Bazzi H
EMBO reports 2021 Feb 3;22(2):e51127

ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.

Evans LT, Anglen T, Scott P, Lukasik K, Loncarek J, Holland AJ
The EMBO journal 2021 Feb 15;40(4):e105106

Exocyst complex mediates recycling of internal cilia.

Rivera-Molina FE, Xi Z, Reales E, Wang B, Toomre D
Current biology : CB 2021 Dec 20;31(24):5580-5589.e5

WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.

Steib E, Laporte MH, Gambarotto D, Olieric N, Zheng C, Borgers S, Olieric V, Le Guennec M, Koll F, Tassin AM, Steinmetz MO, Guichard P, Hamel V
eLife 2020 Sep 18;9

Prolonged mitosis results in structurally aberrant and over-elongated centrioles.

Kong D, Sahabandu N, Sullenberger C, Vásquez-Limeta A, Luvsanjav D, Lukasik K, Loncarek J
The Journal of cell biology 2020 Jun 1;219(6)

Ciliogenesis and Hedgehog signalling are suppressed downstream of KRAS during acinar-ductal metaplasia in mouse.

Bangs FK, Miller P, O'Neill E
Disease models & mechanisms 2020 Jul 30;13(7)

NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling.

Martin-Hurtado A, Martin-Morales R, Robledinos-Antón N, Blanco R, Palacios-Blanco I, Lastres-Becker I, Cuadrado A, Garcia-Gonzalo FR
Scientific reports 2019 Sep 25;9(1):13896

The C7orf43/TRAPPC14 component links the TRAPPII complex to Rabin8 for preciliary vesicle tethering at the mother centriole during ciliogenesis.

Cuenca A, Insinna C, Zhao H, John P, Weiss MA, Lu Q, Walia V, Specht S, Manivannan S, Stauffer J, Peden AA, Westlake CJ
The Journal of biological chemistry 2019 Oct 18;294(42):15418-15434

Cep128 associates with Odf2 to form the subdistal appendage of the centriole.

Kashihara H, Chiba S, Kanno SI, Suzuki K, Yano T, Tsukita S
Genes to cells : devoted to molecular & cellular mechanisms 2019 Mar;24(3):231-243

High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.

Bowler M, Kong D, Sun S, Nanjundappa R, Evans L, Farmer V, Holland A, Mahjoub MR, Sui H, Loncarek J
Nature communications 2019 Mar 1;10(1):993

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

Odabasi E, Gul S, Kavakli IH, Firat-Karalar EN
EMBO reports 2019 Jun;20(6)

RABL2 positively controls localization of GPCRs in mammalian primary cilia.

Dateyama I, Sugihara Y, Chiba S, Ota R, Nakagawa R, Kobayashi T, Itoh H
Journal of cell science 2019 Jan 16;132(2)

Expansion microscopy for the analysis of centrioles and cilia.

Sahabandu N, Kong D, Magidson V, Nanjundappa R, Sullenberger C, Mahjoub MR, Loncarek J
Journal of microscopy 2019 Dec;276(3):145-159

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Huang N, Zhang D, Li F, Chai P, Wang S, Teng J, Chen J
Nature communications 2018 Oct 30;9(1):4511

Oncoprotein CIP2A promotes the disassembly of primary cilia and inhibits glycolytic metabolism.

Jeong AL, Ka HI, Han S, Lee S, Lee EW, Soh SJ, Joo HJ, Sumiyasuren B, Park JY, Lim JS, Park JH, Lee MS, Yang Y
EMBO reports 2018 May;19(5)

Glucose deprivation induces primary cilium formation through mTORC1 inactivation.

Takahashi K, Nagai T, Chiba S, Nakayama K, Mizuno K
Journal of cell science 2018 Jan 8;131(1)

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Ott C, Nachmias D, Adar S, Jarnik M, Sherman S, Birnbaum RY, Lippincott-Schwartz J, Elia N
Scientific reports 2018 Feb 20;8(1):3353

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

Kanie T, Abbott KL, Mooney NA, Plowey ED, Demeter J, Jackson PK
Developmental cell 2017 Jul 10;42(1):22-36.e12

Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis.

Hamel V, Steib E, Hamelin R, Armand F, Borgers S, Flückiger I, Busso C, Olieric N, Sorzano COS, Steinmetz MO, Guichard P, Gönczy P
Current biology : CB 2017 Aug 21;27(16):2486-2498.e6

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

Xu X, Huang S, Zhang B, Huang F, Chi W, Fu J, Wang G, Li S, Jiang Q, Zhang C
Nature communications 2017 Apr 27;8:15164

Genetic link between renal birth defects and congenital heart disease.

San Agustin JT, Klena N, Granath K, Panigrahy A, Stewart E, Devine W, Strittmatter L, Jonassen JA, Liu X, Lo CW, Pazour GJ
Nature communications 2016 Mar 22;7:11103

TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).

Stephen LA, Tawamie H, Davis GM, Tebbe L, Nürnberg P, Nürnberg G, Thiele H, Thoenes M, Boltshauser E, Uebe S, Rompel O, Reis A, Ekici AB, McTeir L, Fraser AM, Hall EA, Mill P, Daudet N, Cross C, Wolfrum U, Jamra RA, Davey MG, Bolz HJ
eLife 2015 Sep 19;4

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using CEP164 antibody 22227-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Other comments: IF
Appendix: 22227-1-AP.pdf

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: WB results of CEP164 (22227-1-AP, 1:500) with RPE1 cells by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.
Other comments: IP
Appendix: 22227-1-AP.pdf

: Show more