22227-1-AP

antibody from Invitrogen Antibodies

Targeting: CEP164 KIAA1052, NPHP15

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

22227-1-AP

Provider

Invitrogen Antibodies

Product name

CEP164 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAGRPLRIG DQLVLEEDYD ETYIPSEQEI LEFAREIGID PIKEPELMWL AREGIVAPLP GEWKPCQDIT GDIYYFNFAN GQSMWDHPCD EHYRSLVIQE RAKLSTSGAI KKK (1-112 aa encoded by BC000602)

Reactivity

Human, Canine

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 22227-1-AP ( CEP164 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF results of CEP164 (22227-1-AP, 1:1000) with HeLa cells (1.5% formaldehyde, 10 min RT) by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF results of CEP164 (22227-1-AP, 1:1000) with HeLa cells (1.5% formaldehyde, 10 min RT) by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MDCK cells using 22227-1-AP (CEP164 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 22227-1-AP (CEP164 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 22227-1-AP (CEP164 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 22227-1-AP ( CEP164 Antibody) at dilution of 1:50 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB results of CEP164 (22227-1-AP, 1:500) with RPE1 cells by Laboratory of Protein Dynamics and Signaling; Center for Cancer Research, National Cancer Institute.