PA5-35370

antibody from Invitrogen Antibodies

Targeting: OAT HOGA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35370

Provider

Invitrogen Antibodies

Product name

OAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OAT in various lysates. Samples were incubated with OAT polyclonal antibody (Product # PA5-35370) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: 293 whole cell lysate; Lane 2: human liver lysate. Predicted band size: 49 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of OAT in 293, 293T cell line, mouse liver and rat liver tissue lysates. Samples were incubated with OAT polyclonal antibody (Product # PA5-35370). Lysates: 35 µg/lane. OAT protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of OAT in mitochondria of A549 cells using an OAT polyclonal antibody (Product # PA5-35370) followed by detection using a fluorescent conjugated secondary antibody (green). Cytoplasmic actin was stained with a fluorescent red phalloidin (7units/mL, 1 h at 37ºC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of OAT in A549 cells. Samples were incubated with OAT polyclonal antibody (Product # PA5-35370) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). OAT immunoreactivity is localized to Mitochondrion significantly.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of OAT in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with OAT polyclonal antibody (Product # PA5-35370) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of OAT in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with OAT polyclonal antibody (Product # PA5-35370) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of OAT in 293 cells (right) compared to a negative control (left) using an OAT polyclonal antibody (Product # PA5-35370) followed by detection using a FITC-conjugated goat-anti-rabbit secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of OAT in 293 cells (right histogram). Samples were incubated with OAT polyclonal antibody (Product # PA5-35370) followed by FITC-conjugated goat-anti-rabbit secondary antibody. Negative control cell (left histogram).