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Western blot
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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-92842 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- OAT Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VKGIQKYKAK IVFAAGNFWG RTLSAISSST DPTSYDGFGP FMPGFDIIPY NDLPALERAL QDPNVAAFMV EPIQGEAGVV VPDPGYLMGV RELCTRHQVL FIADEIQTGL ARTGRWLAVD YENVRPDIVL LGKALSGGLY PVSAVLCDDD IMLTIKPGEH GSTYGGNPLG CRVAIAALEV LEEENLAENA DKLGIILRNE LMKLPSDVVT AVRGKGLLNA IVIKETKDWD AWKVCLRLRD NGLLAKPTHG DIIRFAPPLV IKEDELRESI EIINKTILSF; Positive Samples: SW480, MCF7, 293T, A-549, HepG2, Mouse liver, Mouse lung, Mouse kidney, Rat kidney; Cellular Location: Mitochondrion matrix
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.73 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
Submitted references Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.
Key J, Torres-Odio S, Bach NC, Gispert S, Koepf G, Reichlmeir M, West AP, Prokisch H, Freisinger P, Newman WG, Shalev S, Sieber SA, Wittig I, Auburger G
Cells 2021 Nov 29;10(12)
Cells 2021 Nov 29;10(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of OAT in 200 μg extracts of MCF-7 cells. Samples were precipitated with 3 μg OAT Polyclonal antibody (Product # PA5-92842). Western blot was performed from the immunoprecipitate using OAT Polyclonal antibody (Product # PA5-92842) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of OAT in paraffin-embedded human esophageal cancer. Samples were incubated with OAT Polyclonal antibody (Product # PA5-92842) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of OAT in paraffin-embedded rat kidney. Samples were incubated with OAT Polyclonal antibody (Product # PA5-92842) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of OAT was performed in 200 µg extracts of MCF-7 cells using OAT Polyclonal Antibody (Product # PA5-92842). Western blot was performed from the immunoprecipitate using OAT Polyclonal Antibody.
