LS-C312779

antibody from LSBio

Targeting: KLK1 Klk6

Antibody data

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Validation data

Product number: LS-C312779 - Provider product page
Provider: LSBio
Product name: KLK1 / Kallikrein 1 Antibody (aa182-197) LS-C312779
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Human
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

KLK1 protein structure - LS-C312779 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: WB of KLK1 / Kallikrein 1 antibody. Lane 1: Recombinant Human KLK1 Protein 10ng. Lane 2: Recombinant Human KLK1 Protein 5ng . Lane 3: Recombinant Human KLK1 Protein 2.5ng.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Anti-Kallikrein 1 antibody, Western blotting Lane 1: Recombinant Human KLK1 Protein 10ng Lane 2: Recombinant Human KLK1 Protein 5ng Lane 3: Recombinant Human KLK1 Protein 2. 5ng

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis of KLK1 using anti-KLK1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KLK1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KLK1 at approximately 29KD. The expected band size for KLK1 is at 29KD.