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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A301-454A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIP2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CIP2A by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Hep-G2, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIP2A antibody (Product # A301-454A; lot A301-454A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CIP2A by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Hep-G2, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CIP2A antibody (Product # A301-454A; lot A301-454A-2) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse and rat CIP2A by western blot. Samples: Whole cell lysate (50 µg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CIP2A antibody A301-454A (lot A301-454A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CIP2A by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CIP2A antibody A301-454A used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate. CIP2A was also immunoprecipitated by rabbit anti-CIP2A antibody A301-453A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CIP2A by immunohistochemistry. Sample: FFPE colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A (Product # A301-454A; lot 2) used at a dilution of 1:200 (1 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CIP2A by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CIP2A (Cat. No. A301-454A) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CIP2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CIP2A antibody (Product # A301-454A; lot A301-454A-2) used for IP at 6 µg per reaction. CIP2A was also immunoprecipitated by a previous lot of this antibody (lot A301-454A-1). For blotting immunoprecipitated CIP2A (Product # A301-454A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
