- MA1-46001 - Invitrogen Antibodies CIP2A antibody

Liu Y, Wang Y, Yao D, Chen X, Zhang F, Feng Y, Li X
Genes 2022 Jan 12;13(1)

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CIP2A was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CIP2A Monoclonal Antibody (2G10) (Product # MA1-46001) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti- Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in cytoplasm. Panel e represents low expressing cell line MCF7 with no expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CIP2A was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CIP2A Monoclonal Antibody (2G10) (Product # MA1-46001) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti- Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in cytoplasm. Panel e represents low expressing cell line MCF7 with no expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of CIP2A in formalin fixed paraffin-embedded (FFPE) human colon cancer. Samples were incubated in CIP2A monoclonal antibody (Product # MA1-46001) using a dilution of 1:200. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of CIP2A in 1 x 10^6 CHO (A) and HEK-293 (B) cells. Samples were incubated in CIP2A monoclonal antibody (Product # MA1-46001) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of CIP2A in 1 x 10^6 CHO (A) and HEK-293 (B) cells. Samples were incubated in CIP2A monoclonal antibody (Product # MA1-46001) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.

MA1-46001