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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA1-46001 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIP2A Monoclonal Antibody (2G10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: Hela whole cell extract, antigen standard for KIAA1524 (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G10
- Vial size
- 100 µL
- Concentration
- 1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Diane-35 and Metformin Induce Autophagy and Apoptosis in Polycystic Ovary Syndrome Women with Early-Stage Endometrial Carcinoma.
Liu Y, Wang Y, Yao D, Chen X, Zhang F, Feng Y, Li X
Genes 2022 Jan 12;13(1)
Genes 2022 Jan 12;13(1)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of p90 autoantigen in HeLa whole cell lysate
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CIP2A using a monoclonal antibody (Product # MA1-46001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CIP2A in HeLa whole cell lysate. Sample was incubated in CIP2A monoclonal antibody (Product # MA1-46001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CIP2A in NIH/3T3 cell lysate. Sample was incubated in CIP2A monoclonal antibody (Product # MA1-46001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti- CIP2A Monoclonal Antibody (Product # MA1-46001) and an ~55kDa band corresponding to CIP2A was observed across the cell lines tested except for MCF-7 which is reported to be a low expressor for CIP2A. An uncharacterized band was also observed in MCF-7 and SK-BR-3 cells at ~35kDa. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Raji (Lane 2), MDA-MB-231 (Lane 3), MCF-7 (Lane 4) and SK-BR-3 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution ) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CIP2A was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CIP2A Monoclonal Antibody (2G10) (Product # MA1-46001) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti- Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization in cytoplasm. Panel e represents low expressing cell line MCF7 with no expression. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of CIP2A in 1 x 10^6 CHO (A) and HEK-293 (B) cells. Samples were incubated in CIP2A monoclonal antibody (Product # MA1-46001) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.
