Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00057510-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00057510-M01, RRID:AB_2215484
- Product name
- Mouse Monoclonal Exportin-5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. XPO5 - exportin 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Knock-down of core proteins regulating microRNA biogenesis has no effect on sensitivity of lung cancer cells to ionizing radiation.
MicroRNAs are expressed and processed by human primary macrophages.
Surova O, Akbar NS, Zhivotovsky B
PloS one 2012;7(3):e33134
PloS one 2012;7(3):e33134
MicroRNAs are expressed and processed by human primary macrophages.
Luers AJ, Loudig OD, Berman JW
Cellular immunology 2010;263(1):1-8
Cellular immunology 2010;263(1):1-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Exportin-5 Antibody (2C5-1B3) [H00057510-M01] - Analysis of XPO5 expression in transfected 293T cell line by XPO5 monoclonal antibody (M01), clone 2C5-1B3.Lane 1: XPO5 transfected lysate(136.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Exportin-5 Antibody (2C5-1B3) [H00057510-M01] - XPO5 monoclonal antibody (M01), clone 2C5-1B3 Analysis of XPO5 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Exportin-5 Antibody (2C5-1B3) [H00057510-M01] - Detection limit for recombinant GST tagged XPO5 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Exportin-5 Antibody (2C5-1B3) [H00057510-M01] - Analysis of monoclonal antibody to XPO5 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Exportin-5 Antibody (2C5-1B3) [H00057510-M01] - Analysis of XPO5 transfected lysate using anti-XPO5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with XPO5 MaxPab rabbit polyclonal antibody.