PA5-53789

antibody from Invitrogen Antibodies

Targeting: XPO5 KIAA1291

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-53789

Provider

Invitrogen Antibodies

Product name

XPO5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ILGPLFTYLH MRLSQKWQVI NQRSLLCGED EAADENPESQ EMLEEQLVRM LTREVMDLIT VCCVSKKGAD HSSAPPADGD DEEMMATEVT PSAMAELTDL GKCLMKHEDV CTALLITAFN SLAWKDTLSC Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 86%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.08 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of XPO5 in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a XPO5 Polyclonal Antibody (Product # PA5-53789). Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of XPO5 in human cell line HEK 293 using a XPO5 Polyclonal Antibody (Product # PA5-53789).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of XPO5 using XPO5 Polyclonal Antibody (Product # PA5-53789) (A) shows similar pattern to an independent XPO5 Polyclonal Antibody (B).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of XPO5 in mouse cell line NIH-3T3 and rat cell line NBT-II using a XPO5 Polyclonal Antibody (Product # PA5-53789).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of XPO5 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a XPO5 Polyclonal Antibody (Product # PA5-53789).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of XPO5 in human testis and liver tissues using XPO5 Polyclonal Antibody (Product # PA5-53789). Corresponding XPO5 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of XPO5 in human testis using XPO5 Polyclonal Antibody (Product # PA5-53789) shows high expression.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of XPO5 in human liver using XPO5 Polyclonal Antibody (Product # PA5-53789) shows low expression as expected.