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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [4]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-93224 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XPO5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MRLSQKWQVI NQRSLLCGED EAADENPESQ EMLEEQLVRM LTREVMDLIT VCCVSKKGAD HSSAPPADGD DEEMMATEVT PSAMAELTDL GKCLMKHEDV CTALLITAFN SLAWKDTLSC QRTTSQLCWP LLKQVLSGTL LADAVTWLFT SVLKGLQMHG QHDGCMASLV HLAFQIYEAL RPRYLEIRAV MEQIPEIQKD SLDQFDCKLL NPSLQKVADK RRKDQFKRLI AGCIGKPLGE QFRKEVHIKN LPSLFKKTKP MLETEVLDND GGGLATIFEP; Positive Samples: MCF7, U-251MG, NIH/3T3, HeLa, PC-12, Mouse brain, Rat brain; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 10.47 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of XPO5 was performed in MCF7 cells using XPO5 Polyclonal Antibody (Product # PA5-93224).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of XPO5 in MCF7 cells. Samples were incubated with XPO5 Polyclonal antibody (Product # PA5-93224).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of XPO5 in PC-12 cells. Samples were incubated with XPO5 Polyclonal antibody (Product # PA5-93224) using a dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of XPO5 in MCF7 cells. Samples were incubated with XPO5 Polyclonal antibody (Product # PA5-93224) using a dilution of 1:500 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of XPO5 in 200 μg extracts of HeLa cells. Samples were precipitated with 1 μg XPO5 Polyclonal antibody (Product # PA5-93224). Western blot was performed from the immunoprecipitate using XPO5 Polyclonal antibody (Product # PA5-93224) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of XPO5 was performed in 200 µg extracts of HeLa cells using XPO5 Polyclonal Antibody (Product # PA5-93224). Western blot was performed from the immunoprecipitate using XPO5 Polyclonal Antibody.
