Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MA1-34789 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP A2B1 Monoclonal Antibody (DP3B3)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-34789 detects HNRNP A2/B1 from chicken, human samples.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- DP3B3
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-hnRNP A2B1 Monoclonal Antibody (DP3B3) (Product # MA1-34789) and a 37 and 38 kDa band corresponding to hnRNP A2B1 was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), MCF7 (Lane 4), RAW 264.7 (Lane 5), Mouse Thymus (Lane 6), Rat Thymus (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Both reported isoforms were picked up at the reported molecular weights as seen.