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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [8]
- Immunocytochemistry [3]
- Other assay [2]
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- Product number
- PA5-34939 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP A2B1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12. Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.09 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references YBX-1 mediated sorting of miR-133 into hypoxia/reoxygenation-induced EPC-derived exosomes to increase fibroblast angiogenesis and MEndoT.
Lin F, Zeng Z, Song Y, Li L, Wu Z, Zhang X, Li Z, Ke X, Hu X
Stem cell research & therapy 2019 Aug 23;10(1):263
Stem cell research & therapy 2019 Aug 23;10(1):263
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP A2B1 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate (C) 30 µg HeLa whole cell lysate (D) 30 µg HepG2 whole cell lysate and E) 30 µg A375 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a hnRNP A2B1 polyclonal antibody (Product # PA5-34939) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP A2B1 using A) 30 µg Neuro2A whole cell lysate (B) 30 µg GL261 whole cell lysate (C) 30 µg C8D30 whole cell lysate (D) 30 µg NIH-3T3 whole cell lysate (E) 30 µg BCL-1 whole cell lysate (F) 30 µg Raw264.7 whole cell lysate and G) 30 µg C2C12 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a hnRNP A2B1 polyclonal antibody (Product # PA5-34939) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP A2B1 using 30 µg PC-12 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a hnRNP A2B1 polyclonal antibody (Product # PA5-34939) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of hnRNP A2B1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of hnRNP A2B1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939) at a dilution of 1:10000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939) and a ~37kDa band corresponding to hnRNP A2B1 was observed across cell lines and tissues tested . Nuclear enriched extracts (30 µg lysate) of HEK-293 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), MCF7 (Lane 4), RAW 264.7 (Lane 5), Mouse Thymus (Lane 6), Rat Thymus (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:10000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of hnRNP A2B1 showing staining in the nucleus of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a hnRNP A2B1 polyclonal antibody (Product # PA5-34939) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of hnRNP A2B1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: hnRNP A2B1 Polyclonal Antibody (Product # PA5 34939) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of hnRNP A2B1 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with hnRNP A2B1 Polyclonal Antibody (Product # PA5-34939) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- hnRNPA2B1 antibody immunoprecipitates hnRNPA2B1 protein in IP experiments. IP Sample: 4500 µg A549 whole cell lysate/extract A. Immunoprecipitation of hnRNPA2B1 protein by 2 µg of hnRNPA2B1 antibody (Product # PA5-34939) B. Control with 2 µg of preimmune rabbit IgG The immunoprecipitated hnRNPA2B1 protein was detected by hnRNPA2B1 antibody diluted at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 Measurements of YBX-1, SYNCRIP, and hnRNPA2B1 in EPCs by qRT-PCR ( a - c ) and western blotting ( d ) after 48-h H/R treatment
