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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [3]
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- Product number
- NB120-6102 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-6102, RRID:AB_2117201
- Product name
- Mouse Monoclonal hnRNP A2B1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references N6-methyladenosine regulates the stability of RNA:DNA hybrids in human cells.
Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4: a potential correlation with autocrine signaling.
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
Abakir A, Giles TC, Cristini A, Foster JM, Dai N, Starczak M, Rubio-Roldan A, Li M, Eleftheriou M, Crutchley J, Flatt L, Young L, Gaffney DJ, Denning C, Dalhus B, Emes RD, Gackowski D, Corrêa IR Jr, Garcia-Perez JL, Klungland A, Gromak N, Ruzov A
Nature genetics 2020 Jan;52(1):48-55
Nature genetics 2020 Jan;52(1):48-55
Transcriptional expression of myelin basic protein in oligodendrocytes depends on functional syntaxin 4: a potential correlation with autocrine signaling.
Bijlard M, Klunder B, de Jonge JC, Nomden A, Tyagi S, de Vries H, Hoekstra D, Baron W
Molecular and cellular biology 2015 Feb;35(4):675-87
Molecular and cellular biology 2015 Feb;35(4):675-87
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG
The EMBO journal 2002 Nov 15;21(22):6195-204
The EMBO journal 2002 Nov 15;21(22):6195-204
Decrease in hnRNP A/B expression during erythropoiesis mediates a pre-mRNA splicing switch.
Hou VC, Lersch R, Gee SL, Ponthier JL, Lo AJ, Wu M, Turck CW, Koury M, Krainer AR, Mayeda A, Conboy JG
The EMBO journal 2002 Nov 15;21(22):6195-204
The EMBO journal 2002 Nov 15;21(22):6195-204
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - hnRNP A2B1 expression in HeLa cells. Lane 1= 15 ug of HeLa nuclear extracts Lane 2= Co-IP of hnRNP A2B1. Image and data from confirmed customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-hnRNP-A2/B1 Clone: DP3B3. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. MDBK 5. MDCK 6. CHO
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hnRNP A2B1 Antibody (DP3B3) [NB120-6102] - Whole cell protein from PC12 (lane 1), NIH-3T3 (lane 2), A431 (lane 3) and HeLa (lane 4) was separated by SDS-PAGE and transferred to PVDF membrane. The membrane was probed with Anti-hnRNP A2B1 at 1 ug/ml and detected with an HRP conjugated anti-mouse secondary antibody by chemiluminescence.
