PA5-19530

antibody from Invitrogen Antibodies

Targeting: HNRNPA2B1 HNRPA2B1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19530

Provider

Invitrogen Antibodies

Product name

Anti-hnRNP A2B1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse, chicken, dog and Xenopus laevis based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MCF7 Whole Cell Lysate using Product # PA5-19530, hnRNP A2B1 primary antibody at a dilution of 1 µg/mL. Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human MCF7 cells using Product # PA5-19530, anti-hnRNP A2B1 antibody. The cells were fixed with PFA (4%)for 10 minutes, permabilised PBS-T for 20 minutes and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. A solution of BSA (1%), normal goat serum (10%) and glycine (0.3 M) was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human MCF7 cells using Product # PA5-19530, anti-hnRNP A2B1 antibody. The cells were fixed with PFA (4%)for 10 minutes, permabilised PBS-T for 20 minutes and exposed to the primary antibody at a concentration of 1 µg/mL for 1 hour at room temp. A solution of BSA (1%), normal goat serum (10%) and glycine (0.3 M) was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).