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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB1719 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Kallikrein 8/Neuropsin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Kallikrein 8/Neuropsin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Kallikrein 3, 5, 11, or rhHGFA is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O60259- Isotype
- IgG
- Antibody clone number
- 189004
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Kallikrein 8/Neuropsin in SH-SY5Y Human Cell Line. Kallikrein 8/Neuropsin was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Kallikrein 8/Neuropsin in Human Brain. Kallikrein 8/Neuropsin was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human Kallikrein 8/Neuropsin Monoclonal Antibody (Catalog # MAB1719) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
