Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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- Product number
- AF3730 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse DPPA4 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse DPPA4 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DPPA4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8CCG4
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance.
Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer.
Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency.
Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes.
Stringer JM, Forster SC, Qu Z, Prokopuk L, O'Bryan MK, Gardner DK, White SJ, Adelson D, Western PS
BMC biology 2018 Sep 20;16(1):104
BMC biology 2018 Sep 20;16(1):104
Genomic functions of developmental pluripotency associated factor 4 (Dppa4) in pluripotent stem cells and cancer.
Klein RH, Tung PY, Somanath P, Fehling HJ, Knoepfler PS
Stem cell research 2018 Aug;31:83-94
Stem cell research 2018 Aug;31:83-94
Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Araúzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Schöler HR
Cell stem cell 2018 Aug 2;23(2):266-275.e6
Cell stem cell 2018 Aug 2;23(2):266-275.e6
Hybrid Cellular Metabolism Coordinated by Zic3 and Esrrb Synergistically Enhances Induction of Naive Pluripotency.
Sone M, Morone N, Nakamura T, Tanaka A, Okita K, Woltjen K, Nakagawa M, Heuser JE, Yamada Y, Yamanaka S, Yamamoto T
Cell metabolism 2017 May 2;25(5):1103-1117.e6
Cell metabolism 2017 May 2;25(5):1103-1117.e6
Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes.
Tung PY, Varlakhanova NV, Knoepfler PS
Stem cells (Dayton, Ohio) 2013 Nov;31(11):2330-42
Stem cells (Dayton, Ohio) 2013 Nov;31(11):2330-42
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- DPPA4 in D3 Mouse Cell Line. DPPA4 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3730) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.