NB100-2583

antibody from Novus Biologicals

Targeting: SUPT6H KIAA0162, SPT6H

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-2583

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-2583, RRID:AB_2196405

Product name

Rabbit Polyclonal Spt6 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Spt6 Antibody [NB100-2583] - Detection of Human SUPT6H on HeLa whole cell lysate using NB100-2583. SUPT6H was successfully immunoprecipitated by another rabbit anti-SUPT6H antibody and NB100-2584.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Spt6 Antibody [NB100-2583] - Detection of Human SUPT6H by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SUPT6H antibody NB100-2583 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Spt6 Antibody [NB100-2583] - Sections of human breast carcinoma (left) and mouse squamous cell carcinoma (right).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Spt6 Antibody [NB100-2583] - Samples: FFPE serial sections of human ovarian carcinoma Antibody: Affinity purified rabbit anti- SUPT6H A 300-802A-1 used at a dilution of 0.4ug/ml (left) and used at a dilution of 0.2ug/ml (right) . Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Spt6 Antibody [NB100-2583] - Samples: FFPE serial sections of human ovarian carcinoma Antibody: Affinity purified rabbit anti- SUPT6H A 300-802A-1 used at a dilution of 0.4ug/ml (left) and used at a dilution of 0.2ug/ml (right). Detection: DAB

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Spt6 Antibody [NB100-2583] - Samples: FFPE serial sections of mouse teratoma Antibody: Affinity purified rabbit anti- SUPT6H A 300-802A-1 used at a dilution of 0.4ug/ml (left) and used at a dilution of 0.2ug/ml (right) . Detection: DAB