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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- NB100-135 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-135, RRID:AB_10001286
- Product name
- Rabbit Polyclonal Serotonin N-acetyltransferase Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This is specific for primate AANAT (3,4).
- Reactivity
- Human, Rat, Bovine, Chicken/Avian, Simian, Zebrafish
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Arylalkylamine N-acetyltransferase: "the Timezyme".
Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87).
Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase.
14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin.
cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation.
Klein DC
The Journal of biological chemistry 2007 Feb 16;282(7):4233-7
The Journal of biological chemistry 2007 Feb 16;282(7):4233-7
Melatonin synthesis enzymes in Macaca mulatta: focus on arylalkylamine N-acetyltransferase (EC 2.3.1.87).
Coon SL, Del Olmo E, Young WS 3rd, Klein DC
The Journal of clinical endocrinology and metabolism 2002 Oct;87(10):4699-706
The Journal of clinical endocrinology and metabolism 2002 Oct;87(10):4699-706
Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase.
Iuvone PM, Brown AD, Haque R, Weller J, Zawilska JB, Chaurasia SS, Ma M, Klein DC
Investigative ophthalmology & visual science 2002 Feb;43(2):564-72
Investigative ophthalmology & visual science 2002 Feb;43(2):564-72
14-3-3 Proteins and photoneuroendocrine transduction: role in controlling the daily rhythm in melatonin.
Klein DC, Ganguly S, Coon S, Weller JL, Obsil T, Hickman A, Dyda F
Biochemical Society transactions 2002 Aug;30(4):365-73
Biochemical Society transactions 2002 Aug;30(4):365-73
cAmp regulation of arylalkylamine N-acetyltransferase (AANAT, EC 2.3.1.87): a new cell line (1E7) provides evidence of intracellular AANAT activation.
Coon SL, Weller JL, Korf HW, Namboodiri MA, Rollag M, Klein DC
The Journal of biological chemistry 2001 Jun 29;276(26):24097-107
The Journal of biological chemistry 2001 Jun 29;276(26):24097-107
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Serotonin N-acetyltransferase Antibody [NB100-135] - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.
