A303-453A
antibody from Invitrogen Antibodies
Targeting: SNRNP200
ASCC3L1, BRR2, HELIC2, KIAA0788, RP33, U5-200KD
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- A303-453A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SNRNP200 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse SNRNP200 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), Jurkat (J; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody A303-453A used for WB at 0.04 µg/ml (A) and 0.4 µg/ml (B) and used for IP at 6 µg/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody A303-454A, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SNRNP200 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- SNRNP200 (Cat. No. A303-453A Lot1) used at a dilution of 1:200 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).